biostudies-literatureUnknown167(1)Gebhardt CMalignant transformation of mouse skin by tumor promoters and chemical carcinogens, such as the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA), is a multistage process leading to the formation of squamous cell carcinomas. It has been shown that mice lacking the AP-1 family member c-Fos exhibit an impaired transition from benign to malignant skin tumors. Here, we demonstrate enhanced expression of the small Ras-related GTPase Rab11a after short-term TPA treatment of mouse back skin. Expression of Rab11a in vivo and in vitro critically depended on c-Fos, because TPA application to the back skin of c-Fos-deficient mice and to mouse embryonic fibroblasts did not induce Rab11a mRNA or protein expression. Moreover, dexamethasone, which is a potent inhibitor of AP-1-mediated transactivaThe American journal of pathology243-53https://www.ebi.ac.uk/biostudies/studies/S-EPMC1603444biostudies-literaturec-Fos-dependent induction of the small ras-related GTPase Rab11a in skin carcinogenesis.PMC1603444Richter KHAngel PHess JBreitenbach UFurstenberger GMauch CGebhardt Cfalsec-Fos-dependent induction of the small ras-related GTPase Rab11a in skin carcinogenesis.Malignant transformation of mouse skin by tumor promoters and chemical carcinogens, such as the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA), is a multistage process leading to the formation of squamous cell carcinomas. It has been shown that mice lacking the AP-1 family member c-Fos exhibit an impaired transition from benign to malignant skin tumors. Here, we demonstrate enhanced expression of the small Ras-related GTPase Rab11a after short-term TPA treatment of mouse back skin. Expression of Rab11a in vivo and in vitro critically depended on c-Fos, because TPA application to the back skin of c-Fos-deficient mice and to mouse embryonic fibroblasts did not induce Rab11a mRNA or protein expression. Moreover, dexamethasone, which is a potent inhibitor of AP-1-mediated transactiva2005-01-01T00:00:00Z2005 Jul2026-07-15T03:58:10.958Z2019-03-27T01:46:05ZS-EPMC16034441597296810.1016/S0002-9440(10)62969-010.1016/s0002-9440(10)62969-0