{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["23(14)"],"submitter":["Thumkeo D"],"pubmed_abstract":["Rho-associated kinase (ROCK), including the ROCK-I and ROCK-II isoforms, is a protein kinase involved in signaling from Rho to actin cytoskeleton. However, in vivo functions of each ROCK isoform remain largely unknown. We generated mice deficient in ROCK-II by gene targeting. ROCK-II(-/-) embryos were found at the expected Mendelian frequency until 13.5 days postcoitum, but approximately 90% died thereafter in utero. ROCK-II(-/-) mice of both genders that survived were born runts, subsequently developed without gross abnormality, and were fertile. Whole-mount staining for a knocked-in lacZ reporter gene revealed that ROCK-II was highly expressed in the labyrinth layer of the placenta. Disruption of architecture and extensive thrombus formation were found in the labyrinth layer of ROCK-II(-"],"journal":["Molecular and cellular biology"],"pagination":["5043-55"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC162229"],"repository":["biostudies-literature"],"pubmed_title":["Targeted disruption of the mouse rho-associated kinase 2 gene results in intrauterine growth retardation and fetal death."],"pmcid":["PMC162229"],"pubmed_authors":["Hirose M","Narumiya S","Keel J","Nonomura K","Thumkeo D","Oshima M","Ishizaki T","Oshima H","Taketo MM"],"additional_accession":[]},"is_claimable":false,"name":"Targeted disruption of the mouse rho-associated kinase 2 gene results in intrauterine growth retardation and fetal death.","description":"Rho-associated kinase (ROCK), including the ROCK-I and ROCK-II isoforms, is a protein kinase involved in signaling from Rho to actin cytoskeleton. However, in vivo functions of each ROCK isoform remain largely unknown. We generated mice deficient in ROCK-II by gene targeting. ROCK-II(-/-) embryos were found at the expected Mendelian frequency until 13.5 days postcoitum, but approximately 90% died thereafter in utero. ROCK-II(-/-) mice of both genders that survived were born runts, subsequently developed without gross abnormality, and were fertile. Whole-mount staining for a knocked-in lacZ reporter gene revealed that ROCK-II was highly expressed in the labyrinth layer of the placenta. Disruption of architecture and extensive thrombus formation were found in the labyrinth layer of ROCK-II(-","dates":{"release":"2003-01-01T00:00:00Z","publication":"2003 Jul","modification":"2025-04-19T08:16:13.196Z","creation":"2020-11-07T10:18:27Z"},"accession":"S-EPMC162229","cross_references":{"pubmed":["12832488"],"doi":["10.1128/MCB.23.14.5043-5055.2003","10.1128/mcb.23.14.5043-5055.2003"]}}