biostudies-literatureLarsson ONIAID NIH HHSNHLBI NIH HHS4375-86https://www.ebi.ac.uk/biostudies/studies/S-EPMC1636353biostudies-literatureUnknown34(16)Aberrant activation of the translation initiation machinery is a common property of malignant cells, and is essential for breast carcinoma cells to manifest a malignant phenotype. How does sustained activation of the rate limiting step in protein synthesis so fundamentally alter a cell? In this report, we test the post transcriptional operon theory as a possible mechanism, employing a model system in which apoptosis resistance is conferred on NIH 3T3 cells by ectopic expression of eIF4E. We show (i) there is a set of 255 transcripts that manifest an increase in translational efficiency during eIF4E-mediated escape from apoptosis; (ii) there is a novel prototype 55 nt RNA consensus hairpin structure that is overrepresented in the 5'-untranslated region of translationally activated transcripNucleic acids researchApoptosis resistance downstream of eIF4E: posttranscriptional activation of an anti-apoptotic transcript carrying a consensus hairpin structure.PMC1636353HL073719T32 HL07741P50 HL50152K08 HL074058P01 AI050162R01 HL076779HL076779T32 HL0077411K08-HL074058-01R01 HL073719P01 AI50162Polunovsky VAPeterson MLarsson OLi SBitterman PBLiang ZReilly CSDahlgren CPerlman DMFan DWahlestedt CfalseApoptosis resistance downstream of eIF4E: posttranscriptional activation of an anti-apoptotic transcript carrying a consensus hairpin structure.Aberrant activation of the translation initiation machinery is a common property of malignant cells, and is essential for breast carcinoma cells to manifest a malignant phenotype. How does sustained activation of the rate limiting step in protein synthesis so fundamentally alter a cell? In this report, we test the post transcriptional operon theory as a possible mechanism, employing a model system in which apoptosis resistance is conferred on NIH 3T3 cells by ectopic expression of eIF4E. We show (i) there is a set of 255 transcripts that manifest an increase in translational efficiency during eIF4E-mediated escape from apoptosis; (ii) there is a novel prototype 55 nt RNA consensus hairpin structure that is overrepresented in the 5'-untranslated region of translationally activated transcrip2006-01-01T00:00:00Z20062026-05-02T19:44:40.792Z2019-03-27T01:46:14ZS-EPMC16363531693631410.1093/nar/gkl558