{"database":"biostudies-literature","file_versions":[],"scores":{"citationCount":0,"reanalysisCount":0,"viewCount":47,"searchCount":0},"additional":{"submitter":["Li J"],"funding":["NIDDK NIH HHS"],"pagination":["9476-81"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC170943"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["100(16)"],"pubmed_abstract":["We have examined gene expression in the fat tissue of normal mice at the onset of diet-induced obesity. Insulin-induced gene 1 (insig-1) mRNA rose progressively with a high-fat diet and declined on a restricted diet. Because insig-1 binds sterol regulatory element-binding protein cleavage-activating protein in the endoplasmic reticulum, thereby blocking proteolytic processing required for sterol regulatory element-binding protein activation, we tested its influence on lipogenesis. In differentiating 3T3-L1 cells, insig-1 and -2 rose in parallel with aP2 mRNA during differentiation. The mRNA of the lipogenic transcription factor, carbohydrate response element-binding protein, was undetectable in undifferentiated 3T3-L1 preadipocytes but rose dramatically during differentiation in 25 mM, but not in 5 mM, glucose. Transfection of mouse or human insig-1 into 3T3-L1 preadipocytes completely prevented oil red O staining and blocked upregulation of aP2, peroxisome proliferator-activated receptor gamma2, and carbohydrate response element-binding protein, while reducing down-regulation of preadipocyte factor 1. The results suggest that insig-1 expression restricts lipogenesis in mature adipocytes and blocks differentiation in preadipocytes."],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Insig-1 \"brakes\" lipogenesis in adipocytes and inhibits differentiation of preadipocytes."],"pmcid":["PMC170943"],"funding_grant_id":["DK58398","DK02700","R01 DK002700","P01 DK058398","K08 DK002700"],"pubmed_authors":["Li J","Unger RH","Takaishi K","Cook W","McCorkle SK"],"view_count":["47"],"additional_accession":[]},"is_claimable":false,"name":"Insig-1 \"brakes\" lipogenesis in adipocytes and inhibits differentiation of preadipocytes.","description":"We have examined gene expression in the fat tissue of normal mice at the onset of diet-induced obesity. Insulin-induced gene 1 (insig-1) mRNA rose progressively with a high-fat diet and declined on a restricted diet. Because insig-1 binds sterol regulatory element-binding protein cleavage-activating protein in the endoplasmic reticulum, thereby blocking proteolytic processing required for sterol regulatory element-binding protein activation, we tested its influence on lipogenesis. In differentiating 3T3-L1 cells, insig-1 and -2 rose in parallel with aP2 mRNA during differentiation. The mRNA of the lipogenic transcription factor, carbohydrate response element-binding protein, was undetectable in undifferentiated 3T3-L1 preadipocytes but rose dramatically during differentiation in 25 mM, but not in 5 mM, glucose. Transfection of mouse or human insig-1 into 3T3-L1 preadipocytes completely prevented oil red O staining and blocked upregulation of aP2, peroxisome proliferator-activated receptor gamma2, and carbohydrate response element-binding protein, while reducing down-regulation of preadipocyte factor 1. The results suggest that insig-1 expression restricts lipogenesis in mature adipocytes and blocks differentiation in preadipocytes.","dates":{"release":"2003-01-01T00:00:00Z","publication":"2003 Aug","modification":"2024-11-20T00:30:26.324Z","creation":"2020-11-07T09:48:18Z"},"accession":"S-EPMC170943","cross_references":{"pubmed":["12869692"],"doi":["10.1073/pnas.1133426100"]}}