{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["97(21)"],"submitter":["Fagard M"],"pubmed_abstract":["Introduction of transgene DNA may lead to specific degradation of RNAs that are homologous to the transgene transcribed sequence through phenomena named post-transcriptional gene silencing (PTGS) in plants, quelling in fungi, and RNA interference (RNAi) in animals. It was shown previously that PTGS, quelling, and RNAi require a set of related proteins (SGS2, QDE-1, and EGO-1, respectively). Here we report the isolation of Arabidopsis mutants impaired in PTGS which are affected at the Argonaute1 (AGO1) locus. AGO1 is similar to QDE-2 required for quelling and RDE-1 required for RNAi. Sequencing of ago1 mutants revealed one amino acid essential for PTGS that is also present in QDE-2 and RDE-1 in a highly conserved motif. Taken together, these results confirm the hypothesis that these process"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pagination":["11650-4"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC17255"],"repository":["biostudies-literature"],"pubmed_title":["AGO1, QDE-2, and RDE-1 are related proteins required for post-transcriptional gene silencing in plants, quelling in fungi, and RNA interference in animals."],"pmcid":["PMC17255"],"pubmed_authors":["Boutet S","Morel JB","Bellini C","Vaucheret H","Fagard M"],"additional_accession":[]},"is_claimable":false,"name":"AGO1, QDE-2, and RDE-1 are related proteins required for post-transcriptional gene silencing in plants, quelling in fungi, and RNA interference in animals.","description":"Introduction of transgene DNA may lead to specific degradation of RNAs that are homologous to the transgene transcribed sequence through phenomena named post-transcriptional gene silencing (PTGS) in plants, quelling in fungi, and RNA interference (RNAi) in animals. It was shown previously that PTGS, quelling, and RNAi require a set of related proteins (SGS2, QDE-1, and EGO-1, respectively). Here we report the isolation of Arabidopsis mutants impaired in PTGS which are affected at the Argonaute1 (AGO1) locus. AGO1 is similar to QDE-2 required for quelling and RDE-1 required for RNAi. Sequencing of ago1 mutants revealed one amino acid essential for PTGS that is also present in QDE-2 and RDE-1 in a highly conserved motif. Taken together, these results confirm the hypothesis that these process","dates":{"release":"2000-01-01T00:00:00Z","publication":"2000 Oct","modification":"2025-04-04T22:06:07.477Z","creation":"2019-03-26T22:29:05Z"},"accession":"S-EPMC17255","cross_references":{"pubmed":["11016954"],"doi":["10.1073/pnas.200217597"]}}