biostudies-literatureUnknown96(16)Botuyan MVElongin is a heterotrimeric transcription elongation factor composed of subunits A, B, and C in mammals. Elongin A and C are F-box-containing and SKP1 homologue proteins, respectively, and are therefore of interest for their potential roles in cell cycle-dependent proteolysis. Mammalian elongin C interacts with both elongin A and elongin B, as well as with the von Hippel-Lindau tumor suppressor protein VHL. To investigate the corresponding interactions in yeast, we have utilized NMR spectroscopy combined with ultracentrifugal sedimentation experiments to examine complexes of yeast elongin C (Elc1) with yeast elongin A (Ela1) and two peptides from homologous regions of Ela1 and human VHL. Elc1 alone is a homotetramer composed of subunits with a structured N-terminal region and a dynamicallyProceedings of the National Academy of Sciences of the United States of America9033-8https://www.ebi.ac.uk/biostudies/studies/S-EPMC17727biostudies-literatureBinding of elongin A or a von Hippel-Lindau peptide stabilizes the structure of yeast elongin C.PMC17727Conaway RCBotuyan MVChakrabartty AArrowsmith CHEdwards AMChazin WJConaway JWKoth CMMer GfalseBinding of elongin A or a von Hippel-Lindau peptide stabilizes the structure of yeast elongin C.Elongin is a heterotrimeric transcription elongation factor composed of subunits A, B, and C in mammals. Elongin A and C are F-box-containing and SKP1 homologue proteins, respectively, and are therefore of interest for their potential roles in cell cycle-dependent proteolysis. Mammalian elongin C interacts with both elongin A and elongin B, as well as with the von Hippel-Lindau tumor suppressor protein VHL. To investigate the corresponding interactions in yeast, we have utilized NMR spectroscopy combined with ultracentrifugal sedimentation experiments to examine complexes of yeast elongin C (Elc1) with yeast elongin A (Ela1) and two peptides from homologous regions of Ela1 and human VHL. Elc1 alone is a homotetramer composed of subunits with a structured N-terminal region and a dynamically1999-01-01T00:00:00Z1999 Aug2025-04-04T10:42:03.759Z2019-03-26T22:27:57ZS-EPMC177271043089010.1073/pnas.96.16.9033