biostudies-literatureUnknown19(2)Vu-Trong KThe effects of increased concentration of inorganic phosphate on the biosynthesis of tylosin, the level of the intracellular adenylates, the energy charge, and the activities of enzymes involved in the synthesis of tylonolide precursors were studied in Streptomyces fradiae NRRL 2702. No metabolic response was observed when elevated levels of inorganic phosphate were added in idiophase. Increased initial levels of inorganic phosphate suppressed tylosin production and markedly increased the levels of the adenylates, although the adenylate energy charge was unchanged. Higher growth and glucose uptake rates were also observed. The activities of methylmalonyl-coenzyme A carboxyltransferase (EC 2.1.3.1) and propionyl-coenzyme A carboxylase (EC 6.4.1.3) were suppressed by the increased concentratAntimicrobial agents and chemotherapy209-12https://www.ebi.ac.uk/biostudies/studies/S-EPMC181395biostudies-literatureMetabolic regulation in tylosin-producing Streptomyces fradiae: phosphate control of tylosin biosynthesis.PMC181395Bhuwapathanapun SVu-Trong KGray PPfalseMetabolic regulation in tylosin-producing Streptomyces fradiae: phosphate control of tylosin biosynthesis.The effects of increased concentration of inorganic phosphate on the biosynthesis of tylosin, the level of the intracellular adenylates, the energy charge, and the activities of enzymes involved in the synthesis of tylonolide precursors were studied in Streptomyces fradiae NRRL 2702. No metabolic response was observed when elevated levels of inorganic phosphate were added in idiophase. Increased initial levels of inorganic phosphate suppressed tylosin production and markedly increased the levels of the adenylates, although the adenylate energy charge was unchanged. Higher growth and glucose uptake rates were also observed. The activities of methylmalonyl-coenzyme A carboxyltransferase (EC 2.1.3.1) and propionyl-coenzyme A carboxylase (EC 6.4.1.3) were suppressed by the increased concentrat1981-01-01T00:00:00Z1981 Feb2025-04-04T09:50:03.308Z2025-02-19T04:02:29.492ZS-EPMC181395734755610.1128/AAC.19.2.209