biostudies-literatureUnknown27(6)Linsley PSmicroRNAs (miRNAs) are abundant, approximately 21-nucleotide, noncoding regulatory RNAs. Each miRNA may regulate hundreds of mRNA targets, but the identities of these targets and the processes they regulate are poorly understood. Here we have explored the use of microarray profiling and functional screening to identify targets and biological processes triggered by the transfection of human cells with miRNAs. We demonstrate that a family of miRNAs sharing sequence identity with miRNA-16 (miR-16) negatively regulates cellular growth and cell cycle progression. miR-16-down-regulated transcripts were enriched with genes whose silencing by small interfering RNAs causes an accumulation of cells in G(0)/G(1). Simultaneous silencing of these genes was more effective at blocking cell cycle progressMolecular and cellular biology2240-52https://www.ebi.ac.uk/biostudies/studies/S-EPMC1820501biostudies-literatureTranscripts targeted by the microRNA-16 family cooperatively regulate cell cycle progression.PMC1820501Johnson JMRaymond CKBartz SRCleary MCarleton MBurchard JCummins JMKibukawa MLinsley PSChau NJackson ALLim LSchelter JMartin MMDai HfalseTranscripts targeted by the microRNA-16 family cooperatively regulate cell cycle progression.microRNAs (miRNAs) are abundant, approximately 21-nucleotide, noncoding regulatory RNAs. Each miRNA may regulate hundreds of mRNA targets, but the identities of these targets and the processes they regulate are poorly understood. Here we have explored the use of microarray profiling and functional screening to identify targets and biological processes triggered by the transfection of human cells with miRNAs. We demonstrate that a family of miRNAs sharing sequence identity with miRNA-16 (miR-16) negatively regulates cellular growth and cell cycle progression. miR-16-down-regulated transcripts were enriched with genes whose silencing by small interfering RNAs causes an accumulation of cells in G(0)/G(1). Simultaneous silencing of these genes was more effective at blocking cell cycle progress2007-01-01T00:00:00Z2007 Mar2025-04-05T10:04:26.358Z2019-03-27T02:03:32ZS-EPMC18205011724220510.1128/MCB.02005-0610.1128/mcb.02005-06