biostudies-literatureDemeny MAWorldwide Cancer Researche316https://www.ebi.ac.uk/biostudies/studies/S-EPMC1820849biostudies-literatureUnknown2(3)TFIID plays a role in nucleating RNA polymerase II preinitiation complex assembly on protein-coding genes. TFIID is a multisubunit complex comprised of the TATA box binding protein (TBP) and 14 TBP-associated factors (TAFs). Another class of multiprotein transcriptional regulatory complexes having histone acetyl transferase (HAT) activity, and containing TAFs, includes TFTC, STAGA and the PCAF/GCN5 complex. Looking for as yet undiscovered subunits by a proteomic approach, we had identified TAF8 and SPT7L in human TFTC preparations. Subsequently, however, we demonstrated that TAF8 was not a stable component of TFTC, but that it is present in a small TAF complex (SMAT), containing TAF8, TAF10 and SPT7L, that co-purified with TFTC. Thus, TAF8 is a subunit of both TFIID and SMAT. The latter haPloS oneIdentification of a small TAF complex and its role in the assembly of TAF-containing complexes.PMC182084903-084Kessler PArgentini MRichardot MDemeny MAJanoshazi ASoutoglou ENagy ZScheer ETora LfalseIdentification of a small TAF complex and its role in the assembly of TAF-containing complexes.TFIID plays a role in nucleating RNA polymerase II preinitiation complex assembly on protein-coding genes. TFIID is a multisubunit complex comprised of the TATA box binding protein (TBP) and 14 TBP-associated factors (TAFs). Another class of multiprotein transcriptional regulatory complexes having histone acetyl transferase (HAT) activity, and containing TAFs, includes TFTC, STAGA and the PCAF/GCN5 complex. Looking for as yet undiscovered subunits by a proteomic approach, we had identified TAF8 and SPT7L in human TFTC preparations. Subsequently, however, we demonstrated that TAF8 was not a stable component of TFTC, but that it is present in a small TAF complex (SMAT), containing TAF8, TAF10 and SPT7L, that co-purified with TFTC. Thus, TAF8 is a subunit of both TFIID and SMAT. The latter ha2007-01-01T00:00:00Z2007 Mar2026-05-29T16:45:38.793Z2019-03-26T23:02:18ZS-EPMC18208491737520210.1371/journal.pone.0000316