{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["45(3)"],"submitter":["Oelemann MC"],"pubmed_abstract":["An optimized set of 24 mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) loci, including a discriminatory subset of 15 loci, has recently been defined for the typing of Mycobacterium tuberculosis. Here, we evaluated the performances of this MIRU-VNTR typing system in combination with spoligotyping for the detection of transmission chains in a population-based study comprising 91% of culture-confirmed tuberculosis patients reported in 2003 in Hamburg, Germany. Of the 154 isolates investigated, more than 90% had high IS6110 copy numbers (>/=6). IS6110 restriction fragment length polymorphism (RFLP) typing resulted in 13 clusters, 5 of which had a confirmed epidemiological link. All five, as well as six of the eight IS6110 clusters with no identified epidemi"],"journal":["Journal of clinical microbiology"],"pagination":["691-7"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC1829086"],"repository":["biostudies-literature"],"pubmed_title":["Assessment of an optimized mycobacterial interspersed repetitive- unit-variable-number tandem-repeat typing system combined with spoligotyping for population-based molecular epidemiology studies of tuberculosis."],"pmcid":["PMC1829086"],"pubmed_authors":["Oelemann MC","Haas W","Rusch-Gerdes S","Niemann S","Vatin V","Locht C","Supply P","Diel R"],"additional_accession":[]},"is_claimable":false,"name":"Assessment of an optimized mycobacterial interspersed repetitive- unit-variable-number tandem-repeat typing system combined with spoligotyping for population-based molecular epidemiology studies of tuberculosis.","description":"An optimized set of 24 mycobacterial interspersed repetitive-unit-variable-number tandem-repeat (MIRU-VNTR) loci, including a discriminatory subset of 15 loci, has recently been defined for the typing of Mycobacterium tuberculosis. Here, we evaluated the performances of this MIRU-VNTR typing system in combination with spoligotyping for the detection of transmission chains in a population-based study comprising 91% of culture-confirmed tuberculosis patients reported in 2003 in Hamburg, Germany. Of the 154 isolates investigated, more than 90% had high IS6110 copy numbers (>/=6). IS6110 restriction fragment length polymorphism (RFLP) typing resulted in 13 clusters, 5 of which had a confirmed epidemiological link. All five, as well as six of the eight IS6110 clusters with no identified epidemi","dates":{"release":"2007-01-01T00:00:00Z","publication":"2007 Mar","modification":"2025-04-22T06:31:38.704Z","creation":"2019-03-27T02:03:36Z"},"accession":"S-EPMC1829086","cross_references":{"pubmed":["17192416"],"doi":["10.1128/jcm.01393-06","10.1128/JCM.01393-06"]}}