biostudies-literatureLiao SNHLBI NIH HHS106-20https://www.ebi.ac.uk/biostudies/studies/S-EPMC1852491biostudies-literatureUnknown42(1)<h4>Unlabelled</h4>Our laboratory showed that overexpression of fibroblast growth factor-2 (FGF2) protected the heart against ischemia-reperfusion injury. FGF2 has different protein isoforms (low [LMW] and high [HMW] molecular weight isoforms) produced from alternative translation start sites. However, which FGF2 isoform(s) mediates this cardioprotection, and which signaling pathway (i.e., mitogen-activated protein kinase (MAPK)) elicits FGF2 isoform-induced cardioprotection remains to be elucidated.<h4>Methods and results</h4>Wildtype, Fgf2 KO (absence of all FGF2 isoforms) and FGF2 LMWKO (absence of LMW isoform) hearts were subjected to an ex vivo work-performing heart ischemic model of 60 min ischemia and 120 min reperfusion. There was a significant decrease in the recovery of post-ischJournal of molecular and cellular cardiologyThe cardioprotective effect of the low molecular weight isoform of fibroblast growth factor-2: the role of JNK signaling.PMC1852491R01 HL075633R56 HL075633R01 HL070174Schultz Jel JScott ALiao SDoetschman TPorter DNewman GfalseThe cardioprotective effect of the low molecular weight isoform of fibroblast growth factor-2: the role of JNK signaling.<h4>Unlabelled</h4>Our laboratory showed that overexpression of fibroblast growth factor-2 (FGF2) protected the heart against ischemia-reperfusion injury. FGF2 has different protein isoforms (low [LMW] and high [HMW] molecular weight isoforms) produced from alternative translation start sites. However, which FGF2 isoform(s) mediates this cardioprotection, and which signaling pathway (i.e., mitogen-activated protein kinase (MAPK)) elicits FGF2 isoform-induced cardioprotection remains to be elucidated.<h4>Methods and results</h4>Wildtype, Fgf2 KO (absence of all FGF2 isoforms) and FGF2 LMWKO (absence of LMW isoform) hearts were subjected to an ex vivo work-performing heart ischemic model of 60 min ischemia and 120 min reperfusion. There was a significant decrease in the recovery of post-isch2007-01-01T00:00:00Z2007 Jan2025-04-04T07:10:29.767Z2019-06-06T14:03:40ZS-EPMC18524911715022910.1016/j.yjmcc.2006.10.005