{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["26(4)"],"submitter":["Abdurashidova G"],"pubmed_abstract":["The human DNA replication origin, located in the lamin B2 gene, interacts with the DNA topoisomerases I and II in a cell cycle-modulated manner. The topoisomerases interact in vivo and in vitro with precise bonds ahead of the start sites of bidirectional replication, within the pre-replicative complex region; topoisomerase I is bound in M, early G1 and G1/S border and topoisomerase II in M and the middle of G1. The Orc2 protein competes for the same sites of the origin bound by either topoisomerase in different moments of the cell cycle; furthermore, it interacts on the DNA with topoisomerase II during the assembly of the pre-replicative complex and with DNA-bound topoisomerase I at the G1/S border. Inhibition of topoisomerase I activity abolishes origin firing. Thus, the two topoisomerase"],"journal":["The EMBO journal"],"pagination":["998-1009"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC1852844"],"repository":["biostudies-literature"],"pubmed_title":["Functional interactions of DNA topoisomerases with a human replication origin."],"pmcid":["PMC1852844"],"pubmed_authors":["Sandoval O","Danailov MB","Abdurashidova G","Biamonti G","Radulescu S","Riva S","Zahariev S","Demidovich A","Santamaria L","Falaschi A"],"additional_accession":[]},"is_claimable":false,"name":"Functional interactions of DNA topoisomerases with a human replication origin.","description":"The human DNA replication origin, located in the lamin B2 gene, interacts with the DNA topoisomerases I and II in a cell cycle-modulated manner. The topoisomerases interact in vivo and in vitro with precise bonds ahead of the start sites of bidirectional replication, within the pre-replicative complex region; topoisomerase I is bound in M, early G1 and G1/S border and topoisomerase II in M and the middle of G1. The Orc2 protein competes for the same sites of the origin bound by either topoisomerase in different moments of the cell cycle; furthermore, it interacts on the DNA with topoisomerase II during the assembly of the pre-replicative complex and with DNA-bound topoisomerase I at the G1/S border. Inhibition of topoisomerase I activity abolishes origin firing. Thus, the two topoisomerase","dates":{"release":"2007-01-01T00:00:00Z","publication":"2007 Feb","modification":"2026-07-09T11:00:50.96Z","creation":"2026-07-09T10:31:49.835Z"},"accession":"S-EPMC1852844","cross_references":{"pubmed":["17290216"],"doi":["10.1038/sj.emboj.7601578"]}}