{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Colangeli R"],"funding":["NIAID NIH HHS"],"pagination":["e87"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC1894825"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["3(6)"],"pubmed_abstract":["Multi-drug tolerance is a key phenotypic property that complicates the sterilization of mammals infected with Mycobacterium tuberculosis. Previous studies have established that iniBAC, an operon that confers multi-drug tolerance to M. bovis BCG through an associated pump-like activity, is induced by the antibiotics isoniazid (INH) and ethambutol (EMB). An improved understanding of the functional role of antibiotic-induced genes and the regulation of drug tolerance may be gained by studying the factors that regulate antibiotic-mediated gene expression. An M. smegmatis strain containing a lacZ gene fused to the promoter of M. tuberculosis iniBAC (PiniBAC) was subjected to transposon mutagenesis. Mutants with constitutive expression and increased EMB-mediated induction of PiniBAC::lacZ mapped"],"journal":["PLoS pathogens"],"pubmed_title":["Transcriptional regulation of multi-drug tolerance and antibiotic-induced responses by the histone-like protein Lsr2 in M. tuberculosis."],"pmcid":["PMC1894825"],"funding_grant_id":["R21 AI043268","AI43268","N01-AI-15547","R01 AI043268"],"pubmed_authors":["Sayers B","Jacobs WR","Hazbon MH","Helb D","Peterson SN","Colangeli R","Vilcheze C","Safi H","Sardone I","Lee CG","Alland D","Fleischmann RD","Jones MB"],"additional_accession":[]},"is_claimable":false,"name":"Transcriptional regulation of multi-drug tolerance and antibiotic-induced responses by the histone-like protein Lsr2 in M. tuberculosis.","description":"Multi-drug tolerance is a key phenotypic property that complicates the sterilization of mammals infected with Mycobacterium tuberculosis. Previous studies have established that iniBAC, an operon that confers multi-drug tolerance to M. bovis BCG through an associated pump-like activity, is induced by the antibiotics isoniazid (INH) and ethambutol (EMB). An improved understanding of the functional role of antibiotic-induced genes and the regulation of drug tolerance may be gained by studying the factors that regulate antibiotic-mediated gene expression. An M. smegmatis strain containing a lacZ gene fused to the promoter of M. tuberculosis iniBAC (PiniBAC) was subjected to transposon mutagenesis. Mutants with constitutive expression and increased EMB-mediated induction of PiniBAC::lacZ mapped","dates":{"release":"2007-01-01T00:00:00Z","publication":"2007 Jun","modification":"2025-04-04T12:53:59.701Z","creation":"2019-03-27T02:04:09Z"},"accession":"S-EPMC1894825","cross_references":{"pubmed":["17590082"],"doi":["10.1371/journal.ppat.0030087"]}}