biostudies-literaturePluskota ENIDDK NIH HHSNHLBI NIH HHS3970-8https://www.ebi.ac.uk/biostudies/studies/S-EPMC1895095biostudies-literatureUnknown106(12)High-throughput genomic technology identified an association between a single nucleotide polymorphism (SNP), a proline (P387) rather than the predominant alanine (A387) at position 387 in thrombospondin-4 (TSP-4) and premature myocardial infarction. The inflammatory hypothesis of atherosclerosis invokes a prominent role of leukocytes and cytokines in pathogenesis. As the expression of TSP-4 by vascular cells permits its exposure to circulating leukocytes, the interactions of human neutrophils (polymorphonuclear leukocytes [PMNs]) with both TSP-4 variants were investigated. Phorbol 12-myristate 13-acetate (PMA)-stimulated PMNs adhered and migrated well and equally on the TSP-4 variants. Integrin alpha(M)beta2 was identified as the TSP-4 receptor mediating these responses, and the 3 epidermaBloodMechanism and effect of thrombospondin-4 polymorphisms on neutrophil function.PMC1895095R01 HL 66197K01DK62128P50 HL-077107Pluskota ETopol EJStenina OIKrukovets ISzpak DPlow EFfalseMechanism and effect of thrombospondin-4 polymorphisms on neutrophil function.High-throughput genomic technology identified an association between a single nucleotide polymorphism (SNP), a proline (P387) rather than the predominant alanine (A387) at position 387 in thrombospondin-4 (TSP-4) and premature myocardial infarction. The inflammatory hypothesis of atherosclerosis invokes a prominent role of leukocytes and cytokines in pathogenesis. As the expression of TSP-4 by vascular cells permits its exposure to circulating leukocytes, the interactions of human neutrophils (polymorphonuclear leukocytes [PMNs]) with both TSP-4 variants were investigated. Phorbol 12-myristate 13-acetate (PMA)-stimulated PMNs adhered and migrated well and equally on the TSP-4 variants. Integrin alpha(M)beta2 was identified as the TSP-4 receptor mediating these responses, and the 3 epiderma2005-01-01T00:00:00Z2005 Dec2025-04-22T04:48:59.163Z2019-06-06T14:37:01ZS-EPMC18950951609988510.1182/blood-2005-03-1292