{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["81(11)"],"submitter":["Rodrigue-Gervais IG"],"pubmed_abstract":["The role of peripheral dendritic cells (DCs) in hepatitis C virus (HCV) infection is unclear. To determine if persistent infection exerts an inhibitory pressure on HCV-specific innate responses, we analyzed DC function in blood through quantification of cell-associated HCV RNA levels in conjunction with multiparametric flow cytometry analysis of pathogen recognition receptor-induced cytokine expression. Independently of the serum viral load, fluorescence-activated cell sorter-purified total DCs had a wide range of cell-associated HCV genomic RNA copy numbers (mean log(10), 5.0 per 10(6) cells; range, 4.3 to 5.8). Here we report that for viremic patients with high viral loads in their total DCs, the myeloid DC (MDC) subset displayed impaired expression of interleukin-12 (IL-12) and tumor ne"],"journal":["Journal of virology"],"pagination":["5537-46"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC1900294"],"repository":["biostudies-literature"],"pubmed_title":["Poly(I:C) and lipopolysaccharide innate sensing functions of circulating human myeloid dendritic cells are affected in vivo in hepatitis C virus-infected patients."],"pmcid":["PMC1900294"],"pubmed_authors":["Willems B","Rodrigue-Gervais IG","Sauve D","Sekaly RP","Beaule G","Jouan L","Lamarre D","Bruneau J"],"additional_accession":[]},"is_claimable":false,"name":"Poly(I:C) and lipopolysaccharide innate sensing functions of circulating human myeloid dendritic cells are affected in vivo in hepatitis C virus-infected patients.","description":"The role of peripheral dendritic cells (DCs) in hepatitis C virus (HCV) infection is unclear. To determine if persistent infection exerts an inhibitory pressure on HCV-specific innate responses, we analyzed DC function in blood through quantification of cell-associated HCV RNA levels in conjunction with multiparametric flow cytometry analysis of pathogen recognition receptor-induced cytokine expression. Independently of the serum viral load, fluorescence-activated cell sorter-purified total DCs had a wide range of cell-associated HCV genomic RNA copy numbers (mean log(10), 5.0 per 10(6) cells; range, 4.3 to 5.8). Here we report that for viremic patients with high viral loads in their total DCs, the myeloid DC (MDC) subset displayed impaired expression of interleukin-12 (IL-12) and tumor ne","dates":{"release":"2007-01-01T00:00:00Z","publication":"2007 Jun","modification":"2025-04-03T22:51:51.068Z","creation":"2019-03-27T02:04:15Z"},"accession":"S-EPMC1900294","cross_references":{"pubmed":["17376921"],"doi":["10.1128/jvi.01741-06","10.1128/JVI.01741-06"]}}