{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["2(8)"],"submitter":["Diks SH"],"pubmed_abstract":["<h4>Background</h4>Reversible phosphorylation catalysed by kinases is probably the most important regulatory mechanism in eukaryotes.<h4>Methodology/principal findings</h4>We studied the in vitro phosphorylation of peptide arrays exhibiting the majority of PhosphoBase-deposited protein sequences, by factors in cell lysates from representatives of various branches of the eukaryotic species. We derived a set of substrates from the PhosphoBase whose phosphorylation by cellular extracts is common to the divergent members of different kingdoms and thus may be considered a minimal eukaryotic phosphoproteome. The protein kinases (or kinome) responsible for phosphorylation of these substrates are involved in a variety of processes such as transcription, translation, and cytoskeletal reorganisation"],"journal":["PloS one"],"pagination":["e777"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC1945084"],"repository":["biostudies-literature"],"pubmed_title":["Evidence for a minimal eukaryotic phosphoproteome?"],"pmcid":["PMC1945084"],"pubmed_authors":["Diks SH","van der Sijde M","Peppelenbosch MP","Parikh K","Ritsema T","Joore J"],"additional_accession":[]},"is_claimable":false,"name":"Evidence for a minimal eukaryotic phosphoproteome?","description":"<h4>Background</h4>Reversible phosphorylation catalysed by kinases is probably the most important regulatory mechanism in eukaryotes.<h4>Methodology/principal findings</h4>We studied the in vitro phosphorylation of peptide arrays exhibiting the majority of PhosphoBase-deposited protein sequences, by factors in cell lysates from representatives of various branches of the eukaryotic species. We derived a set of substrates from the PhosphoBase whose phosphorylation by cellular extracts is common to the divergent members of different kingdoms and thus may be considered a minimal eukaryotic phosphoproteome. The protein kinases (or kinome) responsible for phosphorylation of these substrates are involved in a variety of processes such as transcription, translation, and cytoskeletal reorganisation","dates":{"release":"2007-01-01T00:00:00Z","publication":"2007 Aug","modification":"2026-04-08T15:49:15.368Z","creation":"2019-03-26T23:01:17Z"},"accession":"S-EPMC1945084","cross_references":{"pubmed":["17712425"],"doi":["10.1371/journal.pone.0000777"]}}