{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ryder E"],"funding":["Medical Research Council","Biotechnology and Biological Sciences Research Council"],"pagination":["615-29"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2013729"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["177(1)"],"pubmed_abstract":["We describe a second-generation deficiency kit for Drosophila melanogaster composed of molecularly mapped deletions on an isogenic background, covering approximately 77% of the Release 5.1 genome. Using a previously reported collection of FRT-bearing P-element insertions, we have generated 655 new deletions and verified a set of 209 deletion-bearing fly stocks. In addition to deletions, we demonstrate how the P elements may also be used to generate a set of custom inversions and duplications, particularly useful for balancing difficult regions of the genome carrying haplo-insufficient loci. We describe a simple computational resource that facilitates selection of appropriate elements for generating custom deletions. Finally, we provide a computational resource that facilitates selection of"],"journal":["Genetics"],"pubmed_title":["The DrosDel deletion collection: a Drosophila genomewide chromosomal deficiency resource."],"pmcid":["PMC2013729"],"funding_grant_id":["G8225539","G18877"],"pubmed_authors":["Nickel C","Stocker H","Ryder E","Chan YS","Marhold J","Szidonya J","Bautista-Llacer R","Maroy P","Serras F","Morley T","Ekstrom K","Johnson G","Dorner C","Kube M","Kauk A","Webster J","Schenkel H","Hafen E","Reuter G","Punset A","Coulson D","Pflugfelder G","Klimm M","Baisch H","Hugentobler C","Mechler B","Ashburner M","Rasmuson-Lestander A","Drummond J","Apelt C","Gubb D","Russell S","Corominas M","Roote J","Blows F","Pal M","Rudolph T"],"additional_accession":[]},"is_claimable":false,"name":"The DrosDel deletion collection: a Drosophila genomewide chromosomal deficiency resource.","description":"We describe a second-generation deficiency kit for Drosophila melanogaster composed of molecularly mapped deletions on an isogenic background, covering approximately 77% of the Release 5.1 genome. Using a previously reported collection of FRT-bearing P-element insertions, we have generated 655 new deletions and verified a set of 209 deletion-bearing fly stocks. In addition to deletions, we demonstrate how the P elements may also be used to generate a set of custom inversions and duplications, particularly useful for balancing difficult regions of the genome carrying haplo-insufficient loci. We describe a simple computational resource that facilitates selection of appropriate elements for generating custom deletions. Finally, we provide a computational resource that facilitates selection of","dates":{"release":"2007-01-01T00:00:00Z","publication":"2007 Sep","modification":"2025-04-05T08:50:24.227Z","creation":"2019-03-27T02:21:40Z"},"accession":"S-EPMC2013729","cross_references":{"pubmed":["17720900"],"doi":["10.1534/genetics.107.076216"]}}