biostudies-literatureRyder EMedical Research CouncilBiotechnology and Biological Sciences Research Council615-29https://www.ebi.ac.uk/biostudies/studies/S-EPMC2013729biostudies-literatureUnknown177(1)We describe a second-generation deficiency kit for Drosophila melanogaster composed of molecularly mapped deletions on an isogenic background, covering approximately 77% of the Release 5.1 genome. Using a previously reported collection of FRT-bearing P-element insertions, we have generated 655 new deletions and verified a set of 209 deletion-bearing fly stocks. In addition to deletions, we demonstrate how the P elements may also be used to generate a set of custom inversions and duplications, particularly useful for balancing difficult regions of the genome carrying haplo-insufficient loci. We describe a simple computational resource that facilitates selection of appropriate elements for generating custom deletions. Finally, we provide a computational resource that facilitates selection ofGeneticsThe DrosDel deletion collection: a Drosophila genomewide chromosomal deficiency resource.PMC2013729G8225539G18877Nickel CStocker HRyder EChan YSMarhold JSzidonya JBautista-Llacer RMaroy PSerras FMorley TEkstrom KJohnson GDorner CKube MKauk AWebster JSchenkel HHafen EReuter GPunset ACoulson DPflugfelder GKlimm MBaisch HHugentobler CMechler BAshburner MRasmuson-Lestander ADrummond JApelt CGubb DRussell SCorominas MRoote JBlows FPal MRudolph TfalseThe DrosDel deletion collection: a Drosophila genomewide chromosomal deficiency resource.We describe a second-generation deficiency kit for Drosophila melanogaster composed of molecularly mapped deletions on an isogenic background, covering approximately 77% of the Release 5.1 genome. Using a previously reported collection of FRT-bearing P-element insertions, we have generated 655 new deletions and verified a set of 209 deletion-bearing fly stocks. In addition to deletions, we demonstrate how the P elements may also be used to generate a set of custom inversions and duplications, particularly useful for balancing difficult regions of the genome carrying haplo-insufficient loci. We describe a simple computational resource that facilitates selection of appropriate elements for generating custom deletions. Finally, we provide a computational resource that facilitates selection of2007-01-01T00:00:00Z2007 Sep2025-04-05T08:50:24.227Z2019-03-27T02:21:40ZS-EPMC20137291772090010.1534/genetics.107.076216