{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["150(8)"],"submitter":["Hinke SA"],"pubmed_abstract":["<h4>Background and purpose</h4>Two mechanisms have been proposed to explain the insulin-sensitising properties of metformin in peripheral tissues: (a) inhibition of electron transport chain complex I, and (b) activation of the AMP activated protein kinase (AMPK). However the relationship between these mechanisms and their contribution to beta-cell death and dysfunction in vitro, are currently unclear.<h4>Experimental approach</h4>The effects of biguanides (metformin and phenformin) were tested on MIN6 beta-cells and primary FACS-purified rat beta-cells. Cell metabolism was assessed biochemically and by FACS analysis, and correlated with AMPK phosphorylation state and cell viability, with or without fuel substrates.<h4>Key results</h4>In MIN6 cells, metformin reduced mitochondrial complex I"],"journal":["British journal of pharmacology"],"pagination":["1031-43"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2013909"],"repository":["biostudies-literature"],"pubmed_title":["Methyl succinate antagonises biguanide-induced AMPK-activation and death of pancreatic beta-cells through restoration of mitochondrial electron transfer."],"pmcid":["PMC2013909"],"pubmed_authors":["Heimberg H","Cai Y","Van de Casteele M","Martens GA","Hinke SA","Pipeleers D","Finsi J"],"additional_accession":[]},"is_claimable":false,"name":"Methyl succinate antagonises biguanide-induced AMPK-activation and death of pancreatic beta-cells through restoration of mitochondrial electron transfer.","description":"<h4>Background and purpose</h4>Two mechanisms have been proposed to explain the insulin-sensitising properties of metformin in peripheral tissues: (a) inhibition of electron transport chain complex I, and (b) activation of the AMP activated protein kinase (AMPK). However the relationship between these mechanisms and their contribution to beta-cell death and dysfunction in vitro, are currently unclear.<h4>Experimental approach</h4>The effects of biguanides (metformin and phenformin) were tested on MIN6 beta-cells and primary FACS-purified rat beta-cells. Cell metabolism was assessed biochemically and by FACS analysis, and correlated with AMPK phosphorylation state and cell viability, with or without fuel substrates.<h4>Key results</h4>In MIN6 cells, metformin reduced mitochondrial complex I","dates":{"release":"2007-01-01T00:00:00Z","publication":"2007 Apr","modification":"2025-04-04T18:41:52.384Z","creation":"2019-03-27T02:21:40Z"},"accession":"S-EPMC2013909","cross_references":{"pubmed":["17339833"],"doi":["10.1038/sj.bjp.0707189"]}}