{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lang B"],"funding":["Medical Research Council"],"pagination":["6330-7"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2094087"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["35(18)"],"pubmed_abstract":["The global transcriptional regulator H-NS selectively silences bacterial genes associated with pathogenicity and responses to environmental insults. Although there is ample evidence that H-NS binds preferentially to DNA containing curved regions, we show here that a major basis for this selectivity is the presence of a conserved sequence motif in H-NS target transcriptons. We further show that there is a strong tendency for the H-NS binding sites to be clustered, both within operons and in genes contained in the pathogenicity-associated islands. In accordance with previously published findings, we show that these motifs occur in AT-rich regions of DNA. On the basis of these observations, we propose that H-NS silences extensive regions of the bacterial chromosome by binding first to nucleat"],"journal":["Nucleic acids research"],"pubmed_title":["High-affinity DNA binding sites for H-NS provide a molecular basis for selective silencing within proteobacterial genomes."],"pmcid":["PMC2094087"],"funding_grant_id":["MC_U105184288","MC_U105185859"],"pubmed_authors":["Geertz M","Muskhelishvili G","Stella S","Bouffartigues E","Travers A","Gualerzi CO","Pon CL","Lang B","Rimsky S","Mavathur R","Buckle M","Babu MM","Blot N"],"additional_accession":[]},"is_claimable":false,"name":"High-affinity DNA binding sites for H-NS provide a molecular basis for selective silencing within proteobacterial genomes.","description":"The global transcriptional regulator H-NS selectively silences bacterial genes associated with pathogenicity and responses to environmental insults. Although there is ample evidence that H-NS binds preferentially to DNA containing curved regions, we show here that a major basis for this selectivity is the presence of a conserved sequence motif in H-NS target transcriptons. We further show that there is a strong tendency for the H-NS binding sites to be clustered, both within operons and in genes contained in the pathogenicity-associated islands. In accordance with previously published findings, we show that these motifs occur in AT-rich regions of DNA. On the basis of these observations, we propose that H-NS silences extensive regions of the bacterial chromosome by binding first to nucleat","dates":{"release":"2007-01-01T00:00:00Z","publication":"2007","modification":"2026-03-16T16:32:53.145Z","creation":"2019-03-27T02:22:01Z"},"accession":"S-EPMC2094087","cross_references":{"pubmed":["17881364"],"doi":["10.1093/nar/gkm712"]}}