{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sherman MM"],"funding":["NIGMS NIH HHS"],"pagination":["3619-28"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC210103"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["171(7)"],"pubmed_abstract":["Isoprenoid compounds are found in all organisms. In Escherichia coli the isoprene pathway has three distinct branches: the modification of tRNA; the respiratory quinones ubiquinone and menaquinone; and the dolichols, which are long-chain alcohols involved in cell wall biosynthesis. Very little is known about procaryotic isoprene biosynthesis compared with what is known about eucaryote isoprene biosynthesis. This study approached some of the questions about isoprenoid biosynthesis and regulation in procaryotes by isolating and characterizing mutants in E. coli. Mutants were selected by determining their resistance to low levels of aminoglycoside antibiotics, which require an electron transport chain for uptake into bacterial cells. The mutants were characterized with regard to their phenoty"],"journal":["Journal of bacteriology"],"pubmed_title":["Isolation and characterization of isoprene mutants of Escherichia coli."],"pmcid":["PMC210103"],"funding_grant_id":["GM-21328"],"pubmed_authors":["Poulter CD","Petersen LA","Sherman MM"],"additional_accession":[]},"is_claimable":false,"name":"Isolation and characterization of isoprene mutants of Escherichia coli.","description":"Isoprenoid compounds are found in all organisms. In Escherichia coli the isoprene pathway has three distinct branches: the modification of tRNA; the respiratory quinones ubiquinone and menaquinone; and the dolichols, which are long-chain alcohols involved in cell wall biosynthesis. Very little is known about procaryotic isoprene biosynthesis compared with what is known about eucaryote isoprene biosynthesis. This study approached some of the questions about isoprenoid biosynthesis and regulation in procaryotes by isolating and characterizing mutants in E. coli. Mutants were selected by determining their resistance to low levels of aminoglycoside antibiotics, which require an electron transport chain for uptake into bacterial cells. The mutants were characterized with regard to their phenoty","dates":{"release":"1989-01-01T00:00:00Z","publication":"1989 Jul","modification":"2025-04-04T09:50:26.886Z","creation":"2025-02-19T04:02:21.925Z"},"accession":"S-EPMC210103","cross_references":{"pubmed":["2661529"],"doi":["10.1128/jb.171.7.3619-3628.1989"]}}