biostudies-literatureYang SYNIAID NIH HHS2919-28https://www.ebi.ac.uk/biostudies/studies/S-EPMC2118177biostudies-literatureUnknown203(13)It is thought that gene conversion (GCV) and somatic hypermutation (SHM) of immunoglobulin (Ig) genes occur in two steps: the generation of uracils in DNA by activation-induced cytidine deaminase, followed by their subsequent repair by various DNA repair pathways to generate sequence-diversified products. It is not known how either of the two steps is targeted specifically to Ig loci. Because of the tight link between transcription and SHM, we have investigated the role of endogenous Ig light chain (IgL) transcriptional control elements in GCV/SHM in the chicken B cell line DT40. Promoter substitution experiments led to identification of a strong RNA polymerase II promoter incapable of supporting efficient GCV/SHM. This surprising finding indicates that high levels of transcription are notThe Journal of experimental medicineControl of gene conversion and somatic hypermutation by immunoglobulin promoter and enhancer sequences.PMC2118177AI066130R21 AI066130Fugmann SDSchatz DGYang SYfalseControl of gene conversion and somatic hypermutation by immunoglobulin promoter and enhancer sequences.It is thought that gene conversion (GCV) and somatic hypermutation (SHM) of immunoglobulin (Ig) genes occur in two steps: the generation of uracils in DNA by activation-induced cytidine deaminase, followed by their subsequent repair by various DNA repair pathways to generate sequence-diversified products. It is not known how either of the two steps is targeted specifically to Ig loci. Because of the tight link between transcription and SHM, we have investigated the role of endogenous Ig light chain (IgL) transcriptional control elements in GCV/SHM in the chicken B cell line DT40. Promoter substitution experiments led to identification of a strong RNA polymerase II promoter incapable of supporting efficient GCV/SHM. This surprising finding indicates that high levels of transcription are not2006-01-01T00:00:00Z2006 Dec2025-04-21T14:55:31.19Z2019-03-26T23:28:22ZS-EPMC21181771717891910.1084/jem.20061835