biostudies-literatureUnknown104(47)Rogers LDMacrophages use phagocytosis to control the spread of pathogens in the body, to clear apoptotic cells, and to aid in tissue remodeling. The phagosomal membrane is traditionally thought to originate from the plasmalemma and then go through a series of maturation steps involving sequential fusion with endosomal compartments, leading to the formation of a phagolysosome. A recent model suggests that the endoplasmic reticulum (ER) is involved in the maturation as well. Here we use stable isotope labeling and multiple quantitative proteomic approaches to follow the dynamic composition of the maturing phagosome in RAW 264.7 macrophage cells to a greater depth and higher temporal resolution than was previously possible. Analysis of the results suggests that the traditional model of a linear sequenProceedings of the National Academy of Sciences of the United States of America18520-5https://www.ebi.ac.uk/biostudies/studies/S-EPMC2141809biostudies-literatureThe dynamic phagosomal proteome and the contribution of the endoplasmic reticulum.PMC2141809Rogers LDFoster LJfalseThe dynamic phagosomal proteome and the contribution of the endoplasmic reticulum.Macrophages use phagocytosis to control the spread of pathogens in the body, to clear apoptotic cells, and to aid in tissue remodeling. The phagosomal membrane is traditionally thought to originate from the plasmalemma and then go through a series of maturation steps involving sequential fusion with endosomal compartments, leading to the formation of a phagolysosome. A recent model suggests that the endoplasmic reticulum (ER) is involved in the maturation as well. Here we use stable isotope labeling and multiple quantitative proteomic approaches to follow the dynamic composition of the maturing phagosome in RAW 264.7 macrophage cells to a greater depth and higher temporal resolution than was previously possible. Analysis of the results suggests that the traditional model of a linear sequen2007-01-01T00:00:00Z2007 Nov2026-04-29T19:50:49.307Z2019-03-27T02:22:09ZS-EPMC21418091800666010.1073/pnas.0705801104