biostudies-literatureUnknown81(24)Hwang HJIn this study, we investigated changes in protein expression of fish cells induced by infection of infectious pancreatic necrosis virus (IPNV) using two-dimensional electrophoresis and matrix-assisted laser desorption-time of flight proton motive force analysis and identified a novel type of salmon annexin 1 that is induced in fish cells by infection with IPNV. Northern blotting showed that this annexin is overexpressed in IPNV-infected cells compared to control cells, and further analysis revealed that it has a 1,509-bp full-length cDNA sequence with an open reading frame encoding 339 amino acids (GenBank accession no. AY944135). Amino acid sequence analysis revealed that this protein belongs to the annexin 1 subfamily. By applying RNA interference, the mRNA levels of salmon annexin 1 werJournal of virology13816-24https://www.ebi.ac.uk/biostudies/studies/S-EPMC2168874biostudies-literatureIdentification and functional analysis of salmon annexin 1 induced by a virus infection in a fish cell line.PMC2168874Park JWKim HGKim JYChung DKHwang HJLee JMMoon CHfalseIdentification and functional analysis of salmon annexin 1 induced by a virus infection in a fish cell line.In this study, we investigated changes in protein expression of fish cells induced by infection of infectious pancreatic necrosis virus (IPNV) using two-dimensional electrophoresis and matrix-assisted laser desorption-time of flight proton motive force analysis and identified a novel type of salmon annexin 1 that is induced in fish cells by infection with IPNV. Northern blotting showed that this annexin is overexpressed in IPNV-infected cells compared to control cells, and further analysis revealed that it has a 1,509-bp full-length cDNA sequence with an open reading frame encoding 339 amino acids (GenBank accession no. AY944135). Amino acid sequence analysis revealed that this protein belongs to the annexin 1 subfamily. By applying RNA interference, the mRNA levels of salmon annexin 1 wer2007-01-01T00:00:00Z2007 Dec2025-04-21T15:27:53.512Z2019-03-27T02:22:21ZS-EPMC21688741788144210.1128/jvi.02822-0610.1128/JVI.02822-06