biostudies-literatureSchwabish MANIGMS NIH HHS6987-95https://www.ebi.ac.uk/biostudies/studies/S-EPMC2168902biostudies-literatureUnknown27(20)The Swi/Snf nucleosome-remodeling complex is recruited by DNA-binding activator proteins, whereupon it alters chromatin structure to increase preinitiation complex formation and transcription. At the SUC2 promoter, the Swi/Snf complex is required for histone eviction in a manner that is independent of transcriptional activity. Swi/Snf travels through coding regions with elongating RNA polymerase (Pol) II, and swi2 mutants exhibit sensitivity to drugs affecting Pol elongation. In FACT-depleted cells, Swi/Snf is important for internal initiation within coding regions, suggesting that Swi/Snf is important for histone eviction that occurs during Pol II elongation. Taken together, these observations suggest that Swi/Snf is important for histone eviction at enhancers and that it also functions as a Pol II elongation factor.Molecular and cellular biologyThe Swi/Snf complex is important for histone eviction during transcriptional activation and RNA polymerase II elongation in vivo.PMC2168902R01 GM030186GM30186R37 GM030186Schwabish MAStruhl KfalseThe Swi/Snf complex is important for histone eviction during transcriptional activation and RNA polymerase II elongation in vivo.The Swi/Snf nucleosome-remodeling complex is recruited by DNA-binding activator proteins, whereupon it alters chromatin structure to increase preinitiation complex formation and transcription. At the SUC2 promoter, the Swi/Snf complex is required for histone eviction in a manner that is independent of transcriptional activity. Swi/Snf travels through coding regions with elongating RNA polymerase (Pol) II, and swi2 mutants exhibit sensitivity to drugs affecting Pol elongation. In FACT-depleted cells, Swi/Snf is important for internal initiation within coding regions, suggesting that Swi/Snf is important for histone eviction that occurs during Pol II elongation. Taken together, these observations suggest that Swi/Snf is important for histone eviction at enhancers and that it also functions as a Pol II elongation factor.2007-01-01T00:00:00Z2007 Oct2025-04-21T14:52:18.643Z2019-03-27T02:22:21ZS-EPMC21689021770939810.1128/mcb.00717-0710.1128/MCB.00717-07