{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["169(3)"],"submitter":["Delacour D"],"pubmed_abstract":["We have previously reported that 1-benzyl-2-acetamido-2-deoxy-alpha-D-galactopyranoside (GalNAc alpha-O-bn), an inhibitor of glycosylation, perturbed apical biosynthetic trafficking in polarized HT-29 cells suggesting an involvement of a lectin-based mechanism. Here, we have identified galectin-4 as one of the major components of detergent-resistant membranes (DRMs) isolated from HT-29 5M12 cells. Galectin-4 was also found in post-Golgi carrier vesicles. The functional role of galectin-4 in polarized trafficking in HT-29 5M12 cells was studied by using a retrovirus-mediated RNA interference. In galectin-4-depleted HT-29 5M12 cells apical membrane markers accumulated intracellularly. In contrast, basolateral membrane markers were not affected. Moreover, galectin-4 depletion altered the DRM "],"journal":["The Journal of cell biology"],"pagination":["491-501"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2171948"],"repository":["biostudies-literature"],"pubmed_title":["Galectin-4 and sulfatides in apical membrane trafficking in enterocyte-like cells."],"pmcid":["PMC2171948"],"pubmed_authors":["Grard G","Gabius HJ","Delacour D","Le Bivic A","Gouyer V","Maes E","Moreau-Hannedouche O","Zanetta JP","Leteurtre E","Pons A","Simons K","Drobecq H","Andre S","Manninen A","Huet G"],"additional_accession":[]},"is_claimable":false,"name":"Galectin-4 and sulfatides in apical membrane trafficking in enterocyte-like cells.","description":"We have previously reported that 1-benzyl-2-acetamido-2-deoxy-alpha-D-galactopyranoside (GalNAc alpha-O-bn), an inhibitor of glycosylation, perturbed apical biosynthetic trafficking in polarized HT-29 cells suggesting an involvement of a lectin-based mechanism. Here, we have identified galectin-4 as one of the major components of detergent-resistant membranes (DRMs) isolated from HT-29 5M12 cells. Galectin-4 was also found in post-Golgi carrier vesicles. The functional role of galectin-4 in polarized trafficking in HT-29 5M12 cells was studied by using a retrovirus-mediated RNA interference. In galectin-4-depleted HT-29 5M12 cells apical membrane markers accumulated intracellularly. In contrast, basolateral membrane markers were not affected. Moreover, galectin-4 depletion altered the DRM ","dates":{"release":"2005-01-01T00:00:00Z","publication":"2005 May","modification":"2025-04-05T11:09:10.7Z","creation":"2021-02-19T19:54:40Z"},"accession":"S-EPMC2171948","cross_references":{"pubmed":["15883199"],"doi":["10.1083/jcb.200407073"]}}