biostudies-literatureUnknown169(3)Delacour DWe have previously reported that 1-benzyl-2-acetamido-2-deoxy-alpha-D-galactopyranoside (GalNAc alpha-O-bn), an inhibitor of glycosylation, perturbed apical biosynthetic trafficking in polarized HT-29 cells suggesting an involvement of a lectin-based mechanism. Here, we have identified galectin-4 as one of the major components of detergent-resistant membranes (DRMs) isolated from HT-29 5M12 cells. Galectin-4 was also found in post-Golgi carrier vesicles. The functional role of galectin-4 in polarized trafficking in HT-29 5M12 cells was studied by using a retrovirus-mediated RNA interference. In galectin-4-depleted HT-29 5M12 cells apical membrane markers accumulated intracellularly. In contrast, basolateral membrane markers were not affected. Moreover, galectin-4 depletion altered the DRM The Journal of cell biology491-501https://www.ebi.ac.uk/biostudies/studies/S-EPMC2171948biostudies-literatureGalectin-4 and sulfatides in apical membrane trafficking in enterocyte-like cells.PMC2171948Grard GGabius HJDelacour DLe Bivic AGouyer VMaes EMoreau-Hannedouche OZanetta JPLeteurtre EPons ASimons KDrobecq HAndre SManninen AHuet GfalseGalectin-4 and sulfatides in apical membrane trafficking in enterocyte-like cells.We have previously reported that 1-benzyl-2-acetamido-2-deoxy-alpha-D-galactopyranoside (GalNAc alpha-O-bn), an inhibitor of glycosylation, perturbed apical biosynthetic trafficking in polarized HT-29 cells suggesting an involvement of a lectin-based mechanism. Here, we have identified galectin-4 as one of the major components of detergent-resistant membranes (DRMs) isolated from HT-29 5M12 cells. Galectin-4 was also found in post-Golgi carrier vesicles. The functional role of galectin-4 in polarized trafficking in HT-29 5M12 cells was studied by using a retrovirus-mediated RNA interference. In galectin-4-depleted HT-29 5M12 cells apical membrane markers accumulated intracellularly. In contrast, basolateral membrane markers were not affected. Moreover, galectin-4 depletion altered the DRM 2005-01-01T00:00:00Z2005 May2025-04-05T11:09:10.7Z2021-02-19T19:54:40ZS-EPMC21719481588319910.1083/jcb.200407073