{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["35(20)"],"submitter":["Mruk I"],"pubmed_abstract":["Type II restriction-modification (R-M) systems comprise a restriction endonuclease (REase) and a protective methyltransferase (MTase). After R-M genes enter a new cell, MTase must appear before REase or the chromosome will be cleaved. PvuII and some other R-M systems achieve this delay by cotranscribing the REase gene with the gene for an autogenous transcription activator (the controlling or 'C' protein C.PvuII). This study reveals, through in vivo titration, that C.PvuII is not only an activator but also a repressor for its own gene. In other systems, this type of circuit can result in oscillatory behavior. Despite the use of identical, symmetrical C protein-binding sequences (C-boxes) in the left and right operators, C.PvuII showed higher in vitro affinity for O(L) than for O(R), implic"],"journal":["Nucleic acids research"],"pagination":["6935-52"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2175313"],"repository":["biostudies-literature"],"pubmed_title":["Regulatory circuit based on autogenous activation-repression: roles of C-boxes and spacer sequences in control of the PvuII restriction-modification system."],"pmcid":["PMC2175313"],"pubmed_authors":["Mruk I","Blumenthal RM","Rajesh P"],"additional_accession":[]},"is_claimable":false,"name":"Regulatory circuit based on autogenous activation-repression: roles of C-boxes and spacer sequences in control of the PvuII restriction-modification system.","description":"Type II restriction-modification (R-M) systems comprise a restriction endonuclease (REase) and a protective methyltransferase (MTase). After R-M genes enter a new cell, MTase must appear before REase or the chromosome will be cleaved. PvuII and some other R-M systems achieve this delay by cotranscribing the REase gene with the gene for an autogenous transcription activator (the controlling or 'C' protein C.PvuII). This study reveals, through in vivo titration, that C.PvuII is not only an activator but also a repressor for its own gene. In other systems, this type of circuit can result in oscillatory behavior. Despite the use of identical, symmetrical C protein-binding sequences (C-boxes) in the left and right operators, C.PvuII showed higher in vitro affinity for O(L) than for O(R), implic","dates":{"release":"2007-01-01T00:00:00Z","publication":"2007","modification":"2026-04-15T21:02:40.018Z","creation":"2019-03-27T02:22:25Z"},"accession":"S-EPMC2175313","cross_references":{"pubmed":["17933763"],"doi":["10.1093/nar/gkm837"]}}