{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["35(21)"],"submitter":["Tagawa M"],"pubmed_abstract":["Template-directed DNA photoligation has been applied to a method to construct heat-resistant two-dimensional (2D) DNA arrays that can work as scaffolds in bottom-up assembly of functional biomolecules and nano-electronic components. DNA double-crossover AB-staggered (DXAB) tiles were covalently connected by enzyme-free template-directed photoligation, which enables a specific ligation reaction in an extremely tight space and under buffer conditions where no enzymes work efficiently. DNA nanostructures created by self-assembly of the DXAB tiles before and after photoligation have been visualized by high-resolution, tapping mode atomic force microscopy in buffer. The improvement of the heat tolerance of 2D DNA arrays was confirmed by heating and visualizing the DNA nanostructures. The heat-resistant DNA arrays may expand the potential of DNA as functional materials in biotechnology and nanotechnology."],"journal":["Nucleic acids research"],"pagination":["e140"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2175349"],"repository":["biostudies-literature"],"pubmed_title":["Heat-resistant DNA tile arrays constructed by template-directed photoligation through 5-carboxyvinyl-2'-deoxyuridine."],"pmcid":["PMC2175349"],"pubmed_authors":["Sugawara T","Suyama A","Shohda K","Tagawa M","Fujimoto K"],"additional_accession":[]},"is_claimable":false,"name":"Heat-resistant DNA tile arrays constructed by template-directed photoligation through 5-carboxyvinyl-2'-deoxyuridine.","description":"Template-directed DNA photoligation has been applied to a method to construct heat-resistant two-dimensional (2D) DNA arrays that can work as scaffolds in bottom-up assembly of functional biomolecules and nano-electronic components. DNA double-crossover AB-staggered (DXAB) tiles were covalently connected by enzyme-free template-directed photoligation, which enables a specific ligation reaction in an extremely tight space and under buffer conditions where no enzymes work efficiently. DNA nanostructures created by self-assembly of the DXAB tiles before and after photoligation have been visualized by high-resolution, tapping mode atomic force microscopy in buffer. The improvement of the heat tolerance of 2D DNA arrays was confirmed by heating and visualizing the DNA nanostructures. The heat-resistant DNA arrays may expand the potential of DNA as functional materials in biotechnology and nanotechnology.","dates":{"release":"2007-01-01T00:00:00Z","publication":"2007","modification":"2021-02-21T01:20:37Z","creation":"2019-03-27T02:22:25Z"},"accession":"S-EPMC2175349","cross_references":{"pubmed":["17982178"],"doi":["10.1093/nar/gkm872"]}}