biostudies-literatureUnknown198(10)Brdickova NWellcome TrustLymphocyte membrane rafts contain molecules critical for immunoreceptor signaling. Here, we report identification of a new raft-associated adaptor protein LIME (Lck-interacting molecule) expressed predominantly in T lymphocytes. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation. Thus, LIME appears to be involved in regulation of T cell activation by coreceptors.The Journal of experimental medicine1453-62https://www.ebi.ac.uk/biostudies/studies/S-EPMC2194115biostudies-literatureLIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling.PMC2194115Paces JSchraven BHorejsi VKliche SBrdicka TSimeoni LAngelisova PHilgert IBrdickova NHorvath OSpicka JMerten CfalseLIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling.Lymphocyte membrane rafts contain molecules critical for immunoreceptor signaling. Here, we report identification of a new raft-associated adaptor protein LIME (Lck-interacting molecule) expressed predominantly in T lymphocytes. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation. Thus, LIME appears to be involved in regulation of T cell activation by coreceptors.2003-01-01T00:00:00Z2003 Nov2021-02-19T11:56:04Z2019-03-26T23:13:24ZS-EPMC21941151461004610.1084/jem.20031484