biostudies-literatureSaban MRNIDDK NIH HHS204https://www.ebi.ac.uk/biostudies/studies/S-EPMC2212656biostudies-literatureUnknown7<h4>Background</h4>Despite being a mainstay for treating superficial bladder carcinoma and a promising agent for interstitial cystitis, the precise mechanism of Bacillus Calmette-Guerin (BCG) remains poorly understood. It is particularly unclear whether BCG is capable of altering gene expression beyond its well-recognized pro-inflammatory effects and how this relates to its therapeutic efficacy. The objective of this study was to determine differentially expressed genes in the mouse bladder following repeated intravesical BCG therapy.<h4>Methods</h4>Mice were transurethrally instilled with BCG or pyrogen-free on days 1, 7, 14, and 21. Seven days after the last instillation, urothelia along with the submucosa was removed and amplified ds-DNA was prepared from control- and BCG-treated bladdeBMC cancerRepeated BCG treatment of mouse bladder selectively stimulates small GTPases and HLA antigens and inhibits single-spanning uroplakins.PMC22126565 R01 DK066101-02R01 DK066101R01 DK0558285 R01 DK055828-05O'Donnell MAWu XRSaban MRHellmich HLSimpson CLang MLIhnat MASaban RDavis CAfalseRepeated BCG treatment of mouse bladder selectively stimulates small GTPases and HLA antigens and inhibits single-spanning uroplakins.<h4>Background</h4>Despite being a mainstay for treating superficial bladder carcinoma and a promising agent for interstitial cystitis, the precise mechanism of Bacillus Calmette-Guerin (BCG) remains poorly understood. It is particularly unclear whether BCG is capable of altering gene expression beyond its well-recognized pro-inflammatory effects and how this relates to its therapeutic efficacy. The objective of this study was to determine differentially expressed genes in the mouse bladder following repeated intravesical BCG therapy.<h4>Methods</h4>Mice were transurethrally instilled with BCG or pyrogen-free on days 1, 7, 14, and 21. Seven days after the last instillation, urothelia along with the submucosa was removed and amplified ds-DNA was prepared from control- and BCG-treated bladde2007-01-01T00:00:00Z2007 Nov2025-04-04T10:36:11.691Z2019-03-27T02:43:51ZS-EPMC22126561798003010.1186/1471-2407-7-204