{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Das P"],"funding":["NICHD NIH HHS"],"pagination":["331-43"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2234587"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["22(2)"],"pubmed_abstract":["In ruminants, conceptus interferon-tau (IFNT) production is necessary for maintenance of pregnancy. We examined the role of protein kinase A (PKA) in regulating IFNT expression through the activation of Ets2 in JAr choriocarcinoma cells. Although overexpression of the catalytic subunit of PKA or the addition of 8-bromo-cAMP had little ability to up-regulate boIFNT1 reporter constructs on their own, coexpression with Ets2 led to a large increase in gene expression. Progressive truncation of reporter constructs indicated that the site of PKA/Ets2 responsiveness lay in a region of the promoter between -126 and -67, which lacks a cAMP response element but contains the functional Ets2-binding site and an activator protein 1 (AP1) site. Specific mutation of the former reduced the PKA/Ets2 effect"],"journal":["Molecular endocrinology (Baltimore, Md.)"],"pubmed_title":["Combinatorial roles of protein kinase A, Ets2, and 3',5'-cyclic-adenosine monophosphate response element-binding protein-binding protein/p300 in the transcriptional control of interferon-tau expression in a trophoblast cell line."],"pmcid":["PMC2234587"],"funding_grant_id":["R01 HD042201","R01 HD 21896","R01 HD 42201","R01 HD021896"],"pubmed_authors":["Gupta R","Roberts RM","Ezashi T","Das P"],"additional_accession":[]},"is_claimable":false,"name":"Combinatorial roles of protein kinase A, Ets2, and 3',5'-cyclic-adenosine monophosphate response element-binding protein-binding protein/p300 in the transcriptional control of interferon-tau expression in a trophoblast cell line.","description":"In ruminants, conceptus interferon-tau (IFNT) production is necessary for maintenance of pregnancy. We examined the role of protein kinase A (PKA) in regulating IFNT expression through the activation of Ets2 in JAr choriocarcinoma cells. Although overexpression of the catalytic subunit of PKA or the addition of 8-bromo-cAMP had little ability to up-regulate boIFNT1 reporter constructs on their own, coexpression with Ets2 led to a large increase in gene expression. Progressive truncation of reporter constructs indicated that the site of PKA/Ets2 responsiveness lay in a region of the promoter between -126 and -67, which lacks a cAMP response element but contains the functional Ets2-binding site and an activator protein 1 (AP1) site. Specific mutation of the former reduced the PKA/Ets2 effect","dates":{"release":"2008-01-01T00:00:00Z","publication":"2008 Feb","modification":"2025-06-01T01:34:33.089Z","creation":"2025-06-01T01:34:33.089Z"},"accession":"S-EPMC2234587","cross_references":{"pubmed":["17975022"],"doi":["10.1210/me.2007-0300"]}}