biostudies-literatureUnknown3(2)Blanco S<h4>Background</h4>Cellular biological responses to specific stimulation are determined by a balance among signaling pathways. Protein interactions are likely to modulate these pathways. Vaccinia-related kinase-2 (VRK2) is a novel human kinase that can modulate different signaling pathways.<h4>Principal findings</h4>We report that in vivo, the activity of JIP1-JNK complexes is downregulated by VRK2 in response to interleukin-1beta. Also the reduction of endogenous VRK2 with shRNA increases the transcriptional response to IL-1beta. The JIP1 scaffold protein assembles three consecutive members of a given MAPK pathway forming signaling complexes and their signal can be modulated by interactions with regulatory proteins that remain to be identified. Knocking-down JIP1 with siRNA resulted in elPloS onee1660https://www.ebi.ac.uk/biostudies/studies/S-EPMC2243017biostudies-literatureModulation of interleukin-1 transcriptional response by the interaction between VRK2 and the JIP1 scaffold protein.PMC2243017Sanz-Garcia MSantos CRBlanco SLazo PAfalseModulation of interleukin-1 transcriptional response by the interaction between VRK2 and the JIP1 scaffold protein.<h4>Background</h4>Cellular biological responses to specific stimulation are determined by a balance among signaling pathways. Protein interactions are likely to modulate these pathways. Vaccinia-related kinase-2 (VRK2) is a novel human kinase that can modulate different signaling pathways.<h4>Principal findings</h4>We report that in vivo, the activity of JIP1-JNK complexes is downregulated by VRK2 in response to interleukin-1beta. Also the reduction of endogenous VRK2 with shRNA increases the transcriptional response to IL-1beta. The JIP1 scaffold protein assembles three consecutive members of a given MAPK pathway forming signaling complexes and their signal can be modulated by interactions with regulatory proteins that remain to be identified. Knocking-down JIP1 with siRNA resulted in el2008-01-01T00:00:00Z2008 Feb2025-04-04T14:11:28.904Z2019-03-26T23:02:35ZS-EPMC22430171828620710.1371/journal.pone.0001660