biostudies-literatureUnknown105(9)Lieu ZZThe transmembrane precursor of tumor necrosis factor-alpha (TNF) exits the trans-Golgi network (TGN) in tubular carriers for subsequent trafficking and delivery to the cell surface; however, the molecular machinery responsible for Golgi export is unknown. We previously reported that members of the TGN golgin family are associated with subdomains and tubules of the TGN. Here, we show that the TGN golgin, p230/golgin-245 (p230), is essential for intracellular trafficking and cell surface delivery of TNF in transfected HeLa cells and activated macrophages. Live-cell imaging revealed that TNF transport from the TGN is mediated selectively by tubules and carriers marked by p230. Significantly, LPS activation of macrophages resulted in a dramatic increase of p230-labeled tubules and carriers emeProceedings of the National Academy of Sciences of the United States of America3351-6https://www.ebi.ac.uk/biostudies/studies/S-EPMC2265200biostudies-literatureA trans-Golgi network golgin is required for the regulated secretion of TNF in activated macrophages in vivo.PMC2265200Gleeson PAStow JLLa Gruta NLLock JGLieu ZZHammond LAfalseA trans-Golgi network golgin is required for the regulated secretion of TNF in activated macrophages in vivo.The transmembrane precursor of tumor necrosis factor-alpha (TNF) exits the trans-Golgi network (TGN) in tubular carriers for subsequent trafficking and delivery to the cell surface; however, the molecular machinery responsible for Golgi export is unknown. We previously reported that members of the TGN golgin family are associated with subdomains and tubules of the TGN. Here, we show that the TGN golgin, p230/golgin-245 (p230), is essential for intracellular trafficking and cell surface delivery of TNF in transfected HeLa cells and activated macrophages. Live-cell imaging revealed that TNF transport from the TGN is mediated selectively by tubules and carriers marked by p230. Significantly, LPS activation of macrophages resulted in a dramatic increase of p230-labeled tubules and carriers eme2008-01-01T00:00:00Z2008 Mar2025-04-19T16:49:12.803Z2019-03-27T02:44:18ZS-EPMC22652001830893010.1073/pnas.0800137105