{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Roehm PC"],"funding":["NIDDK NIH HHS","NIDCD NIH HHS"],"pagination":["376-87"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2265381"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["37(2)"],"pubmed_abstract":["The effect of membrane electrical activity on spiral ganglion neuron (SGN) neurite growth remains unknown despite its relevance to cochlear implant technology. We demonstrate that membrane depolarization delays the initial formation and inhibits the subsequent extension of cultured SGN neurites. This inhibition depends directly on the level of depolarization with higher levels of depolarization causing retraction of existing neurites. Cultured SGNs express subunits for L-type, N-type, and P/Q type voltage-gated calcium channels (VGCCs) and removal of extracellular Ca(2+) or treatment with a combination of L-type, N-type, and P/Q-type VGCC antagonists rescues SGN neurite growth under depolarizing conditions. By measuring the fluorescence intensity of SGNs loaded with the fluorogenic calpain substrate t-butoxy carbonyl-Leu-Met-chloromethylaminocoumarin (20 microM), we demonstrate that depolarization activates calpains. Calpeptin (15 microM), a calpain inhibitor, prevents calpain activation by depolarization and rescues neurite growth in depolarized SGNs suggesting that calpain activation contributes to the inhibition of neurite growth by depolarization."],"journal":["Molecular and cellular neurosciences"],"pubmed_title":["Membrane depolarization inhibits spiral ganglion neurite growth via activation of multiple types of voltage sensitive calcium channels and calpain."],"pmcid":["PMC2265381"],"funding_grant_id":["K08 DC006211-01A1","K08 DC006211-04","K08 DC006211-03","K08 DC006211-05","K08 DC006211-02","K08 DC006211","L30 DC007091-01","L30 DC007091","P30 DK054759","R01 DC002961","R01 DC02961","P30 DK 54759","L30 DC007091-02"],"pubmed_authors":["Xu N","Hansen MR","Green SH","Woodson EA","Roehm PC"],"additional_accession":[]},"is_claimable":false,"name":"Membrane depolarization inhibits spiral ganglion neurite growth via activation of multiple types of voltage sensitive calcium channels and calpain.","description":"The effect of membrane electrical activity on spiral ganglion neuron (SGN) neurite growth remains unknown despite its relevance to cochlear implant technology. We demonstrate that membrane depolarization delays the initial formation and inhibits the subsequent extension of cultured SGN neurites. This inhibition depends directly on the level of depolarization with higher levels of depolarization causing retraction of existing neurites. Cultured SGNs express subunits for L-type, N-type, and P/Q type voltage-gated calcium channels (VGCCs) and removal of extracellular Ca(2+) or treatment with a combination of L-type, N-type, and P/Q-type VGCC antagonists rescues SGN neurite growth under depolarizing conditions. By measuring the fluorescence intensity of SGNs loaded with the fluorogenic calpain substrate t-butoxy carbonyl-Leu-Met-chloromethylaminocoumarin (20 microM), we demonstrate that depolarization activates calpains. Calpeptin (15 microM), a calpain inhibitor, prevents calpain activation by depolarization and rescues neurite growth in depolarized SGNs suggesting that calpain activation contributes to the inhibition of neurite growth by depolarization.","dates":{"release":"2008-01-01T00:00:00Z","publication":"2008 Feb","modification":"2021-02-20T17:20:20Z","creation":"2019-03-27T02:44:19Z"},"accession":"S-EPMC2265381","cross_references":{"pubmed":["18055215"],"doi":["10.1016/j.mcn.2007.10.014"]}}