biostudies-literatureXiong SNIGMS NIH HHS12260-5https://www.ebi.ac.uk/biostudies/studies/S-EPMC22819biostudies-literatureUnknown95(21)To investigate the regulation of the human fatty acid synthase gene by the thyroid hormone triiodothyronine, various constructs of the human fatty acid synthase promoter and the luciferase reporter gene were transfected in combination with plasmids expressing the thyroid hormone and the retinoid X receptors in HepG2 cells. The reporter gene was activated 25-fold by the thyroid hormone in the presence of the thyroid hormone receptor. When both the thyroid hormone and the retinoid X receptors were expressed in HepG2 cells, there was about a 100-fold increase in reporter gene expression. 5'-Deletion analysis disclosed two thyroid hormone response elements, TRE1 (nucleotides -870 to -650) and TRE2 (nucleotides -272 to -40), in the human fatty acid synthase promoter. The presence of thyroid horProceedings of the National Academy of Sciences of the United States of AmericaIdentification of thyroid hormone response elements in the human fatty acid synthase promoter.PMC22819F32 GM019091GM-19091Wakil SJChirala SSHsu MHXiong SfalseIdentification of thyroid hormone response elements in the human fatty acid synthase promoter.To investigate the regulation of the human fatty acid synthase gene by the thyroid hormone triiodothyronine, various constructs of the human fatty acid synthase promoter and the luciferase reporter gene were transfected in combination with plasmids expressing the thyroid hormone and the retinoid X receptors in HepG2 cells. The reporter gene was activated 25-fold by the thyroid hormone in the presence of the thyroid hormone receptor. When both the thyroid hormone and the retinoid X receptors were expressed in HepG2 cells, there was about a 100-fold increase in reporter gene expression. 5'-Deletion analysis disclosed two thyroid hormone response elements, TRE1 (nucleotides -870 to -650) and TRE2 (nucleotides -272 to -40), in the human fatty acid synthase promoter. The presence of thyroid hor1998-01-01T00:00:00Z1998 Oct2025-04-04T09:50:13.68Z2025-02-19T04:02:28.623ZS-EPMC22819977047410.1073/pnas.95.21.12260