{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["McCrann DJ"],"funding":["NIDDK NIH HHS","NHLBI NIH HHS"],"pagination":["4092-5"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2288720"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["111(8)"],"pubmed_abstract":["The level of survivin was reported to be scarce in mouse megakaryocytes (MKs) compared with erythroid cells. Considering this finding and previously reported in vitro data showing decreased MK ploidy upon retroviral-mediated overexpression of survivin, we sought to examine whether ectopic survivin expression in the MK lineage might alter ploidy level in vivo. Here we report the generation of 2 tissue specific hematopoietic transgenic mouse models, one expressing survivin in both the erythroid and MK lineages and the other expressing survivin solely in the MK lineage. Survivin protein overexpression was confirmed in MKs and erythrocytes. Surprisingly, analysis of both transgenic mouse lines showed no detectable changes in MK number, ploidy level, and platelet and erythrocyte counts, as compared with control mice. We conclude that elevated survivin expression does not alter MK/erythroid lineage development and that elevated survivin, alone, does not interfere with MK ploidy in vivo."],"journal":["Blood"],"pubmed_title":["Survivin overexpression alone does not alter megakaryocyte ploidy nor interfere with erythroid/megakaryocytic lineage development in transgenic mice."],"pmcid":["PMC2288720"],"funding_grant_id":["R56 DK074693","DK074693","T32 HL007969","R01 DK074693","HL80442","R01 HL080442","R01 DK074693-02"],"pubmed_authors":["Liu H","Nguyen HG","McCrann DJ","Yezefski T","Papadantonakis N","Wen Q","Crispino JD","Ravid K"],"additional_accession":[]},"is_claimable":false,"name":"Survivin overexpression alone does not alter megakaryocyte ploidy nor interfere with erythroid/megakaryocytic lineage development in transgenic mice.","description":"The level of survivin was reported to be scarce in mouse megakaryocytes (MKs) compared with erythroid cells. Considering this finding and previously reported in vitro data showing decreased MK ploidy upon retroviral-mediated overexpression of survivin, we sought to examine whether ectopic survivin expression in the MK lineage might alter ploidy level in vivo. Here we report the generation of 2 tissue specific hematopoietic transgenic mouse models, one expressing survivin in both the erythroid and MK lineages and the other expressing survivin solely in the MK lineage. Survivin protein overexpression was confirmed in MKs and erythrocytes. Surprisingly, analysis of both transgenic mouse lines showed no detectable changes in MK number, ploidy level, and platelet and erythrocyte counts, as compared with control mice. We conclude that elevated survivin expression does not alter MK/erythroid lineage development and that elevated survivin, alone, does not interfere with MK ploidy in vivo.","dates":{"release":"2008-01-01T00:00:00Z","publication":"2008 Apr","modification":"2021-03-11T08:22:07Z","creation":"2019-03-27T02:44:38Z"},"accession":"S-EPMC2288720","cross_references":{"pubmed":["18245663"],"doi":["10.1182/blood-2007-11-122150"]}}