{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["180(6)"],"submitter":["Goetz JG"],"pubmed_abstract":["Both tyrosine-phosphorylated caveolin-1 (pY14Cav1) and GlcNAc-transferase V (Mgat5) are linked with focal adhesions (FAs); however, their function in this context is unknown. Here, we show that galectin-3 binding to Mgat5-modified N-glycans functions together with pY14Cav1 to stabilize focal adhesion kinase (FAK) within FAs, and thereby promotes FA disassembly and turnover. Expression of the Mgat5/galectin lattice alone induces FAs and cell spreading. However, FAK stabilization in FAs also requires expression of pY14Cav1. In cells lacking the Mgat5/galectin lattice, pY14Cav1 is not sufficient to promote FAK stabilization, FA disassembly, and turnover. In human MDA-435 cancer cells, Cav1 expression, but not mutant Y14FCav1, stabilizes FAK exchange and stimulates de novo FA formation in prot"],"journal":["The Journal of cell biology"],"pagination":["1261-75"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2290850"],"repository":["biostudies-literature"],"pubmed_title":["Concerted regulation of focal adhesion dynamics by galectin-3 and tyrosine-phosphorylated caveolin-1."],"pmcid":["PMC2290850"],"pubmed_authors":["Goetz JG","Lajoie P","Kojic LD","Scudamore T","Joshi B","Strugnell SS","Nabi IR"],"additional_accession":[]},"is_claimable":false,"name":"Concerted regulation of focal adhesion dynamics by galectin-3 and tyrosine-phosphorylated caveolin-1.","description":"Both tyrosine-phosphorylated caveolin-1 (pY14Cav1) and GlcNAc-transferase V (Mgat5) are linked with focal adhesions (FAs); however, their function in this context is unknown. Here, we show that galectin-3 binding to Mgat5-modified N-glycans functions together with pY14Cav1 to stabilize focal adhesion kinase (FAK) within FAs, and thereby promotes FA disassembly and turnover. Expression of the Mgat5/galectin lattice alone induces FAs and cell spreading. However, FAK stabilization in FAs also requires expression of pY14Cav1. In cells lacking the Mgat5/galectin lattice, pY14Cav1 is not sufficient to promote FAK stabilization, FA disassembly, and turnover. In human MDA-435 cancer cells, Cav1 expression, but not mutant Y14FCav1, stabilizes FAK exchange and stimulates de novo FA formation in prot","dates":{"release":"2008-01-01T00:00:00Z","publication":"2008 Mar","modification":"2025-04-22T07:06:07.865Z","creation":"2019-03-26T23:20:00Z"},"accession":"S-EPMC2290850","cross_references":{"pubmed":["18347068"],"doi":["10.1083/jcb.200709019"]}}