biostudies-literatureUnknown180(6)Goetz JGBoth tyrosine-phosphorylated caveolin-1 (pY14Cav1) and GlcNAc-transferase V (Mgat5) are linked with focal adhesions (FAs); however, their function in this context is unknown. Here, we show that galectin-3 binding to Mgat5-modified N-glycans functions together with pY14Cav1 to stabilize focal adhesion kinase (FAK) within FAs, and thereby promotes FA disassembly and turnover. Expression of the Mgat5/galectin lattice alone induces FAs and cell spreading. However, FAK stabilization in FAs also requires expression of pY14Cav1. In cells lacking the Mgat5/galectin lattice, pY14Cav1 is not sufficient to promote FAK stabilization, FA disassembly, and turnover. In human MDA-435 cancer cells, Cav1 expression, but not mutant Y14FCav1, stabilizes FAK exchange and stimulates de novo FA formation in protThe Journal of cell biology1261-75https://www.ebi.ac.uk/biostudies/studies/S-EPMC2290850biostudies-literatureConcerted regulation of focal adhesion dynamics by galectin-3 and tyrosine-phosphorylated caveolin-1.PMC2290850Goetz JGLajoie PKojic LDScudamore TJoshi BStrugnell SSNabi IRfalseConcerted regulation of focal adhesion dynamics by galectin-3 and tyrosine-phosphorylated caveolin-1.Both tyrosine-phosphorylated caveolin-1 (pY14Cav1) and GlcNAc-transferase V (Mgat5) are linked with focal adhesions (FAs); however, their function in this context is unknown. Here, we show that galectin-3 binding to Mgat5-modified N-glycans functions together with pY14Cav1 to stabilize focal adhesion kinase (FAK) within FAs, and thereby promotes FA disassembly and turnover. Expression of the Mgat5/galectin lattice alone induces FAs and cell spreading. However, FAK stabilization in FAs also requires expression of pY14Cav1. In cells lacking the Mgat5/galectin lattice, pY14Cav1 is not sufficient to promote FAK stabilization, FA disassembly, and turnover. In human MDA-435 cancer cells, Cav1 expression, but not mutant Y14FCav1, stabilizes FAK exchange and stimulates de novo FA formation in prot2008-01-01T00:00:00Z2008 Mar2025-04-22T07:06:07.865Z2019-03-26T23:20:00ZS-EPMC22908501834706810.1083/jcb.200709019