biostudies-literatureUnknown64(Pt 2)Kurz Malpha-DsbA1 is one of two DsbA homologues encoded by the Gram-negative alpha-proteobacterium Wolbachia pipientis, an endosymbiont that can behave as a reproductive parasite in insects and as a mutualist in medically important filarial nematodes. The alpha-DsbA1 protein is thought to be important for the folding and secretion of Wolbachia proteins involved in the induction of reproductive distortions. Crystals of native and SeMet alpha-DsbA1 were grown by vapour diffusion and belong to the monoclinic space group C2, with unit-cell parameters a = 71.4, b = 49.5, c = 69.3 A, beta = 107.0 degrees and one molecule in the asymmetric unit (44% solvent content). X-ray data were recorded from native crystals to a resolution of 2.01 A using a copper anode and data from SeMet alpha-DsbA1 crystals werActa crystallographica. Section F, Structural biology and crystallization communications94-7https://www.ebi.ac.uk/biostudies/studies/S-EPMC2374166biostudies-literatureCrystallization and preliminary diffraction analysis of a DsbA homologue from Wolbachia pipientis.PMC2374166Jarrott RIturbe-Ormaetxe IO'Neill SLByriel KAHeras BMartin JLKurz MfalseCrystallization and preliminary diffraction analysis of a DsbA homologue from Wolbachia pipientis.alpha-DsbA1 is one of two DsbA homologues encoded by the Gram-negative alpha-proteobacterium Wolbachia pipientis, an endosymbiont that can behave as a reproductive parasite in insects and as a mutualist in medically important filarial nematodes. The alpha-DsbA1 protein is thought to be important for the folding and secretion of Wolbachia proteins involved in the induction of reproductive distortions. Crystals of native and SeMet alpha-DsbA1 were grown by vapour diffusion and belong to the monoclinic space group C2, with unit-cell parameters a = 71.4, b = 49.5, c = 69.3 A, beta = 107.0 degrees and one molecule in the asymmetric unit (44% solvent content). X-ray data were recorded from native crystals to a resolution of 2.01 A using a copper anode and data from SeMet alpha-DsbA1 crystals wer2008-01-01T00:00:00Z2008 Feb2025-04-21T18:05:50.586Z2019-03-27T00:13:56ZS-EPMC23741661825905810.1107/S174430910800005510.1107/s1744309108000055