{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["88(4)"],"submitter":["Fletcher GC"],"pubmed_abstract":["hAG-2 and hAG-3 are recently discovered human homologues of the secreted Xenopus laevis proteins XAG-1/2 (AGR-1/2) that are expressed in the cement gland, an ectodermal organ in the head associated with anteroposterior fate determination during early development. Although the roles of hAG-2 and hAG-3 in mammalian cells are unknown, both proteins share a high degree of protein sequence homology and lie adjacent to one another on chromosome 7p21. hAG-2 mRNA expression has previously been demonstrated in oestrogen receptor (ER)-positive cell lines. In this study, we have used real-time quantitative RT - PCR analysis and immunohistochemistry on tissue microarrays to demonstrate concordant expression of hAG-2 and hAG-3 mRNA and protein in breast tumour tissues. Tumour expression of both genes c"],"journal":["British journal of cancer"],"pagination":["579-85"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2377166"],"repository":["biostudies-literature"],"pubmed_title":["hAG-2 and hAG-3, human homologues of genes involved in differentiation, are associated with oestrogen receptor-positive breast tumours and interact with metastasis gene C4.4a and dystroglycan."],"pmcid":["PMC2377166"],"pubmed_authors":["Fletcher GC","Daviet L","Harris AL","Terrett JA","Tyson K","Patel S","Parekh R","Legrain P","Adam PJ","Schenker M","Loader JA"],"additional_accession":[]},"is_claimable":false,"name":"hAG-2 and hAG-3, human homologues of genes involved in differentiation, are associated with oestrogen receptor-positive breast tumours and interact with metastasis gene C4.4a and dystroglycan.","description":"hAG-2 and hAG-3 are recently discovered human homologues of the secreted Xenopus laevis proteins XAG-1/2 (AGR-1/2) that are expressed in the cement gland, an ectodermal organ in the head associated with anteroposterior fate determination during early development. Although the roles of hAG-2 and hAG-3 in mammalian cells are unknown, both proteins share a high degree of protein sequence homology and lie adjacent to one another on chromosome 7p21. hAG-2 mRNA expression has previously been demonstrated in oestrogen receptor (ER)-positive cell lines. In this study, we have used real-time quantitative RT - PCR analysis and immunohistochemistry on tissue microarrays to demonstrate concordant expression of hAG-2 and hAG-3 mRNA and protein in breast tumour tissues. Tumour expression of both genes c","dates":{"release":"2003-01-01T00:00:00Z","publication":"2003 Feb","modification":"2025-04-25T23:19:11.368Z","creation":"2019-03-27T02:45:04Z"},"accession":"S-EPMC2377166","cross_references":{"pubmed":["12592373"],"doi":["10.1038/sj.bjc.6600740"]}}