{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Salahpour A"],"funding":["NIDA NIH HHS","Howard Hughes Medical Institute","NINDS NIH HHS"],"pagination":["4405-10"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2393812"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["105(11)"],"pubmed_abstract":["The dopamine transporter (DAT) plays a key role in the regulation of dopaminergic signaling wherein it controls both the spatial and temporal actions of dopamine. Here we evaluated the behavioral and neurochemical consequences of increased DAT function by generating DAT transgenic mice (DAT-tg) that overexpress the transporter. These mice were generated by pronuclear injection of a bacterial artificial chromosome containing the mouse DAT locus, yielding an anatomical expression pattern of DAT-tg identical to WT. In DAT-tg mice there is a 3-fold increase in the levels of total and membrane-expressed DAT, but synaptic plasma membrane fractions of DAT-tg mice show only a 30% increase in transporter levels. Functional studies reveal that in the DAT-tg animals there is a 50% increase in the rat"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["Increased amphetamine-induced hyperactivity and reward in mice overexpressing the dopamine transporter."],"pmcid":["PMC2393812"],"funding_grant_id":["R01 NS038879","R01 NS019576","K01 DA017703","NS-19576","NS-38879"],"pubmed_authors":["Medvedev IO","Caron MG","Salahpour A","Ramsey AJ","Sesack SR","Kile B","Gainetdinov RR","Sotnikova TD","Nicholls PJ","Murphy K","Wightman RM","Holmstrand E","Wong L","Ghisi V"],"additional_accession":[]},"is_claimable":false,"name":"Increased amphetamine-induced hyperactivity and reward in mice overexpressing the dopamine transporter.","description":"The dopamine transporter (DAT) plays a key role in the regulation of dopaminergic signaling wherein it controls both the spatial and temporal actions of dopamine. Here we evaluated the behavioral and neurochemical consequences of increased DAT function by generating DAT transgenic mice (DAT-tg) that overexpress the transporter. These mice were generated by pronuclear injection of a bacterial artificial chromosome containing the mouse DAT locus, yielding an anatomical expression pattern of DAT-tg identical to WT. In DAT-tg mice there is a 3-fold increase in the levels of total and membrane-expressed DAT, but synaptic plasma membrane fractions of DAT-tg mice show only a 30% increase in transporter levels. Functional studies reveal that in the DAT-tg animals there is a 50% increase in the rat","dates":{"release":"2008-01-01T00:00:00Z","publication":"2008 Mar","modification":"2026-03-16T16:25:44.223Z","creation":"2019-03-27T00:15:11Z"},"accession":"S-EPMC2393812","cross_references":{"pubmed":["18347339"],"doi":["10.1073/pnas.0707646105"]}}