{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Gajduskova P"],"funding":["NCI NIH HHS"],"pagination":["R120"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2394771"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(6)"],"pubmed_abstract":["<h4>Background</h4>Amplifications, regions of focal high-level copy number change, lead to overexpression of oncogenes or drug resistance genes in tumors. Their presence is often associated with poor prognosis; however, the use of amplification as a mechanism for overexpression of a particular gene in tumors varies. To investigate the influence of genome position on propensity to amplify, we integrated a mutant form of the gene encoding dihydrofolate reductase into different positions in the human genome, challenged cells with methotrexate and then studied the genomic alterations arising in drug resistant cells.<h4>Results</h4>We observed site-specific differences in methotrexate sensitivity, amplicon organization and amplification frequency. One site was uniquely associated with a signifi"],"journal":["Genome biology"],"pubmed_title":["Genome position and gene amplification."],"pmcid":["PMC2394771"],"funding_grant_id":["R33 CA094407","CA90421","CA94407","R01 CA090421"],"pubmed_authors":["Tokuyasu T","Gajduskova P","Kwek S","Roydasgupta R","Pinkel D","Fridlyand J","Snijders AM","Albertson DG"],"additional_accession":[]},"is_claimable":false,"name":"Genome position and gene amplification.","description":"<h4>Background</h4>Amplifications, regions of focal high-level copy number change, lead to overexpression of oncogenes or drug resistance genes in tumors. Their presence is often associated with poor prognosis; however, the use of amplification as a mechanism for overexpression of a particular gene in tumors varies. To investigate the influence of genome position on propensity to amplify, we integrated a mutant form of the gene encoding dihydrofolate reductase into different positions in the human genome, challenged cells with methotrexate and then studied the genomic alterations arising in drug resistant cells.<h4>Results</h4>We observed site-specific differences in methotrexate sensitivity, amplicon organization and amplification frequency. One site was uniquely associated with a signifi","dates":{"release":"2007-01-01T00:00:00Z","publication":"2007","modification":"2026-04-30T14:57:44.364Z","creation":"2026-04-07T16:04:47.253Z"},"accession":"S-EPMC2394771","cross_references":{"pubmed":["17584934"],"doi":["10.1186/gb-2007-8-6-r120"]}}