{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["36(9)"],"submitter":["Ciais D"],"funding":["Wellcome Trust"],"pubmed_abstract":["Microarray analyses of mRNAs over-expressed in strains lacking the nuclear exosome component Rrp6 identified the transcript encoding the ARE-binding protein Cth2, which functions in cytoplasmic mRNA stability. Subsequent northern analyses revealed that exosome mutants accumulate a 3'-extended transcript at the expense of the mature CTH2 mRNA. The 3' ends of the CTH2 mRNA were mapped to a [GU(3-5)](5) repeat, unlike any previously characterized polyadenylation site. CTH2 mRNA accumulation was not inhibited by mutations in 3'-cleavage and polyadenylation factors, Rna14, Rna15 and Pap1, which block accumulation of other mRNAs. The 3'-extended CTH2 pre-mRNA strongly accumulated in strains with mutations in the TRAMP4 polyadenylation complex or the Nrd1/Nab3/Sen1 complex, and contains multiple "],"journal":["Nucleic acids research"],"pagination":["3075-84"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2396412"],"repository":["biostudies-literature"],"pubmed_title":["The mRNA encoding the yeast ARE-binding protein Cth2 is generated by a novel 3' processing pathway."],"pmcid":["PMC2396412"],"pubmed_authors":["Tollervey D","Bohnsack MT","Ciais D"],"additional_accession":[]},"is_claimable":false,"name":"The mRNA encoding the yeast ARE-binding protein Cth2 is generated by a novel 3' processing pathway.","description":"Microarray analyses of mRNAs over-expressed in strains lacking the nuclear exosome component Rrp6 identified the transcript encoding the ARE-binding protein Cth2, which functions in cytoplasmic mRNA stability. Subsequent northern analyses revealed that exosome mutants accumulate a 3'-extended transcript at the expense of the mature CTH2 mRNA. The 3' ends of the CTH2 mRNA were mapped to a [GU(3-5)](5) repeat, unlike any previously characterized polyadenylation site. CTH2 mRNA accumulation was not inhibited by mutations in 3'-cleavage and polyadenylation factors, Rna14, Rna15 and Pap1, which block accumulation of other mRNAs. The 3'-extended CTH2 pre-mRNA strongly accumulated in strains with mutations in the TRAMP4 polyadenylation complex or the Nrd1/Nab3/Sen1 complex, and contains multiple ","dates":{"release":"2008-01-01T00:00:00Z","publication":"2008 May","modification":"2025-04-04T21:06:25.035Z","creation":"2019-03-27T00:15:15Z"},"accession":"S-EPMC2396412","cross_references":{"pubmed":["18400782"],"doi":["10.1093/nar/gkn160"]}}