{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Rardin MJ"],"funding":["NIGMS NIH HHS","PHS HHS"],"pagination":["15440-50"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2397459"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["283(22)"],"pubmed_abstract":["Although large-scale approaches have identified numerous mitochondrial phosphoproteins, little is known about the mitochondrial kinases and phosphatases that regulate these phosphoproteins. Here, we identify two members of the atypical dual specificity phosphatases (DSP), DSP18 and DSP21, that are localized in mitochondria. Although DSP18 is widely expressed in several mammalian tissues, DSP21 is selectively expressed in the testes. We demonstrate that DSP18 and DSP21 are targeted to mitochondria by cryptic internal localization signals. Subfractionation of mitochondria demonstrated that DSP18 is located in the intermembrane space as a peripheral membrane protein of the inner membrane. In contrast, subfractionation of rat testis mitochondria revealed DSP21 is localized to the matrix as a p"],"journal":["The Journal of biological chemistry"],"pubmed_title":["Dual specificity phosphatases 18 and 21 target to opposing sides of the mitochondrial inner membrane."],"pmcid":["PMC2397459"],"funding_grant_id":["T32 GM007752","NIH 18849","2T32 GM 07752-25"],"pubmed_authors":["Pagliarini DJ","Wiley SE","Murphy AN","Rardin MJ","Dixon JE"],"additional_accession":[]},"is_claimable":false,"name":"Dual specificity phosphatases 18 and 21 target to opposing sides of the mitochondrial inner membrane.","description":"Although large-scale approaches have identified numerous mitochondrial phosphoproteins, little is known about the mitochondrial kinases and phosphatases that regulate these phosphoproteins. Here, we identify two members of the atypical dual specificity phosphatases (DSP), DSP18 and DSP21, that are localized in mitochondria. Although DSP18 is widely expressed in several mammalian tissues, DSP21 is selectively expressed in the testes. We demonstrate that DSP18 and DSP21 are targeted to mitochondria by cryptic internal localization signals. Subfractionation of mitochondria demonstrated that DSP18 is located in the intermembrane space as a peripheral membrane protein of the inner membrane. In contrast, subfractionation of rat testis mitochondria revealed DSP21 is localized to the matrix as a p","dates":{"release":"2008-01-01T00:00:00Z","publication":"2008 May","modification":"2025-04-18T14:20:05.401Z","creation":"2019-03-27T00:15:18Z"},"accession":"S-EPMC2397459","cross_references":{"pubmed":["18385140"],"doi":["10.1074/jbc.M709547200"]}}