biostudies-literatureDostalek MNIDDK NIH HHSNIEHS NIH HHSNCI NIH HHSNIGMS NIH HHS17147-57https://www.ebi.ac.uk/biostudies/studies/S-EPMC2427356biostudies-literatureUnknown283(25)Reactive oxygen species (ROS) and oxidative stress have been considered in a variety of disease models, and cytochrome P450 (P450) enzymes have been suggested to be a source of ROS. Induction of P450s by phenobarbital (PB), beta-naphthoflavone (betaNF), or clofibrate in a mouse model increased ROS parameters in the isolated liver microsomes, but isoniazid treatment did not. However, when F(2)-isoprostanes (F(2)-IsoPs) were measured in tissues and urine, PB showed the strongest effect and betaNF had a measurable but weaker effect. The same trend was seen when an Nfr2-based transgene reporter sensitive to ROS was analyzed in the mice. This pattern had been seen earlier with F(2)-IsoPs both in vitro and in vivo with rats (Dostalek, M., Brooks, J. D., Hardy, K. D., Milne, G. L., Moore, M. M., The Journal of biological chemistryDevelopment of oxidative stress by cytochrome P450 induction in rodents is selective for barbiturates and related to loss of pyridine nucleotide-dependent protective systems.PMC2427356P50 GM16431R01 ES007462-13R01 DK48831P01 ES13125P30 ES000267R01 ES007462R01 ES08089R01 ES10042R37 CA090426R01 ES07462Ding XMartin MVDostalek MHardy KDMorrow JDGu JJohnson JAGonzalez FJChen CJohnson DAGuengerich FPMilne GLfalseDevelopment of oxidative stress by cytochrome P450 induction in rodents is selective for barbiturates and related to loss of pyridine nucleotide-dependent protective systems.Reactive oxygen species (ROS) and oxidative stress have been considered in a variety of disease models, and cytochrome P450 (P450) enzymes have been suggested to be a source of ROS. Induction of P450s by phenobarbital (PB), beta-naphthoflavone (betaNF), or clofibrate in a mouse model increased ROS parameters in the isolated liver microsomes, but isoniazid treatment did not. However, when F(2)-isoprostanes (F(2)-IsoPs) were measured in tissues and urine, PB showed the strongest effect and betaNF had a measurable but weaker effect. The same trend was seen when an Nfr2-based transgene reporter sensitive to ROS was analyzed in the mice. This pattern had been seen earlier with F(2)-IsoPs both in vitro and in vivo with rats (Dostalek, M., Brooks, J. D., Hardy, K. D., Milne, G. L., Moore, M. M., 2008-01-01T00:00:00Z2008 Jun2025-05-29T21:52:18.826Z2025-05-29T21:52:18.826ZS-EPMC24273561844297410.1074/jbc.M80244720010.1074/jbc.m802447200