biostudies-literatureUnknown2(7)Galvan-Moroyoqui JM<h4>Background</h4>Mixed intestinal infections with Entamoeba histolytica, Entamoeba dispar and bacteria with exacerbated manifestations of disease are common in regions where amoebiasis is endemic. However, amoeba-bacteria interactions remain largely unexamined.<h4>Methodology</h4>Trophozoites of E. histolytica and E. dispar were co-cultured with enteropathogenic bacteria strains Escherichia coli (EPEC), Shigella dysenteriae and a commensal Escherichia coli. Amoebae that phagocytosed bacteria were tested for a cytopathic effect on epithelial cell monolayers. Cysteine proteinase activity, adhesion and cell surface concentration of Gal/GalNAc lectin were analyzed in amoebae showing increased virulence. Structural and functional changes and induction of IL-8 expression were determined in epiPLoS neglected tropical diseasese266https://www.ebi.ac.uk/biostudies/studies/S-EPMC2447883biostudies-literatureThe interplay between Entamoeba and enteropathogenic bacteria modulates epithelial cell damage.PMC2447883Franco EMeza IDel Carmen Dominguez-Robles MGalvan-Moroyoqui JMfalseThe interplay between Entamoeba and enteropathogenic bacteria modulates epithelial cell damage.<h4>Background</h4>Mixed intestinal infections with Entamoeba histolytica, Entamoeba dispar and bacteria with exacerbated manifestations of disease are common in regions where amoebiasis is endemic. However, amoeba-bacteria interactions remain largely unexamined.<h4>Methodology</h4>Trophozoites of E. histolytica and E. dispar were co-cultured with enteropathogenic bacteria strains Escherichia coli (EPEC), Shigella dysenteriae and a commensal Escherichia coli. Amoebae that phagocytosed bacteria were tested for a cytopathic effect on epithelial cell monolayers. Cysteine proteinase activity, adhesion and cell surface concentration of Gal/GalNAc lectin were analyzed in amoebae showing increased virulence. Structural and functional changes and induction of IL-8 expression were determined in epi2008-01-01T00:00:00Z2008 Jul2025-04-04T18:33:48.182Z2019-03-27T00:15:45ZS-EPMC24478831864851710.1371/journal.pntd.0000266