biostudies-literatureCarlson CBNCRR NIH HHSNHLBI NIH HHS20069-76https://www.ebi.ac.uk/biostudies/studies/S-EPMC2459288biostudies-literatureUnknown283(29)Thrombospondins (THBSs) are multimodular, secreted proteins characterized by a signature domain comprising a unique set of 13 calcium-binding repeats flanked by epidermal growth factor (EGF)-like and lectin-like modules. A polymorphism that changes a conserved Asn to Ser at residue 700 in the most N-terminal calcium-binding repeat of THBS-1 (repeat 1C) is found in 8-10% of European populations and has been linked to increased risk of premature coronary artery disease. The Ser substitution leads to altered stability in the EGF-like and wire modules of the THBS-1 signature domain as assessed by differential scanning calorimetry carried out in 2 mm or 200 mum calcium. Studies of the melting profiles of the THBS-2 signature domain proteins with Asn or Ser at position 702 (homologous to 700 in The Journal of biological chemistryInfluences of the N700S thrombospondin-1 polymorphism on protein structure and stability.PMC2459288HL 07899HL 54462S10 RR 13790Liu YKeck JLCarlson CBMosher DFfalseInfluences of the N700S thrombospondin-1 polymorphism on protein structure and stability.Thrombospondins (THBSs) are multimodular, secreted proteins characterized by a signature domain comprising a unique set of 13 calcium-binding repeats flanked by epidermal growth factor (EGF)-like and lectin-like modules. A polymorphism that changes a conserved Asn to Ser at residue 700 in the most N-terminal calcium-binding repeat of THBS-1 (repeat 1C) is found in 8-10% of European populations and has been linked to increased risk of premature coronary artery disease. The Ser substitution leads to altered stability in the EGF-like and wire modules of the THBS-1 signature domain as assessed by differential scanning calorimetry carried out in 2 mm or 200 mum calcium. Studies of the melting profiles of the THBS-2 signature domain proteins with Asn or Ser at position 702 (homologous to 700 in 2008-01-01T00:00:00Z2008 Jul2026-05-01T15:41:24.91Z2019-03-27T00:15:48ZS-EPMC24592881849967410.1074/jbc.M80022320010.1074/jbc.m800223200