{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["27(15)"],"submitter":["Du F"],"pubmed_abstract":["The contractile vacuole (CV) system is the osmoregulatory organelle required for survival for many free-living cells under hypotonic conditions. We identified a new CV regulator, Disgorgin, a TBC-domain-containing protein, which translocates to the CV membrane at the late stage of CV charging and regulates CV-plasma membrane fusion and discharging. disgorgin(-) cells produce large CVs due to impaired CV-plasma membrane fusion. Disgorgin is a specific GAP for Rab8A-GTP, which also localizes to the CV and whose hydrolysis is required for discharging. We demonstrate that Drainin, a previously identified TBC-domain-containing protein, lies upstream from Disgorgin in this pathway. Unlike Disgorgin, Drainin lacks GAP activity but functions as a Rab11A effector. The BEACH family proteins LvsA and"],"journal":["The EMBO journal"],"pagination":["2064-76"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2516880"],"repository":["biostudies-literature"],"pubmed_title":["Regulation of contractile vacuole formation and activity in Dictyostelium."],"pmcid":["PMC2516880"],"pubmed_authors":["Briggs S","Shen Z","Sun B","De Lozanne A","Du F","Firtel RA","Edwards K"],"additional_accession":[]},"is_claimable":false,"name":"Regulation of contractile vacuole formation and activity in Dictyostelium.","description":"The contractile vacuole (CV) system is the osmoregulatory organelle required for survival for many free-living cells under hypotonic conditions. We identified a new CV regulator, Disgorgin, a TBC-domain-containing protein, which translocates to the CV membrane at the late stage of CV charging and regulates CV-plasma membrane fusion and discharging. disgorgin(-) cells produce large CVs due to impaired CV-plasma membrane fusion. Disgorgin is a specific GAP for Rab8A-GTP, which also localizes to the CV and whose hydrolysis is required for discharging. We demonstrate that Drainin, a previously identified TBC-domain-containing protein, lies upstream from Disgorgin in this pathway. Unlike Disgorgin, Drainin lacks GAP activity but functions as a Rab11A effector. The BEACH family proteins LvsA and","dates":{"release":"2008-01-01T00:00:00Z","publication":"2008 Aug","modification":"2026-03-16T16:25:45.188Z","creation":"2019-03-27T00:16:12Z"},"accession":"S-EPMC2516880","cross_references":{"pubmed":["18636095"],"doi":["10.1038/emboj.2008.131"]}}