{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["74(15)"],"submitter":["Beutin L"],"pubmed_abstract":["Shiga toxin 2e (Stx2e)-producing strains from food (n = 36), slaughtered pigs (n = 25), the environment (n = 21), diseased pigs (n = 19), and humans (n = 9) were investigated for production of Stx2e by enzyme-linked immunosorbent assay, for virulence markers by PCR, and for their serotypes to evaluate their role as potential human pathogens. Stx2e production was low in 64% of all 110 strains. Stx2e production was inducible by mitomycin C but differed considerably between strains. Analysis by nucleotide sequencing and transcription of stx(2e) genes in high- and low-Stx2e-producing strains showed that toxin production correlated with transcription rates of stx(2e) genes. DNA sequences specific for the int, Q, dam, and S genes of the stx(2e) bacteriophage P27 were found in 109 strains, indica"],"journal":["Applied and environmental microbiology"],"pagination":["4806-16"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC2519352"],"repository":["biostudies-literature"],"pubmed_title":["Evaluation of major types of Shiga toxin 2E-producing Escherichia coli bacteria present in food, pigs, and the environment as potential pathogens for humans."],"pmcid":["PMC2519352"],"pubmed_authors":["Kruger U","Strauch E","Beutin L","Miko A","Krause G","Martin A"],"additional_accession":[]},"is_claimable":false,"name":"Evaluation of major types of Shiga toxin 2E-producing Escherichia coli bacteria present in food, pigs, and the environment as potential pathogens for humans.","description":"Shiga toxin 2e (Stx2e)-producing strains from food (n = 36), slaughtered pigs (n = 25), the environment (n = 21), diseased pigs (n = 19), and humans (n = 9) were investigated for production of Stx2e by enzyme-linked immunosorbent assay, for virulence markers by PCR, and for their serotypes to evaluate their role as potential human pathogens. Stx2e production was low in 64% of all 110 strains. Stx2e production was inducible by mitomycin C but differed considerably between strains. Analysis by nucleotide sequencing and transcription of stx(2e) genes in high- and low-Stx2e-producing strains showed that toxin production correlated with transcription rates of stx(2e) genes. DNA sequences specific for the int, Q, dam, and S genes of the stx(2e) bacteriophage P27 were found in 109 strains, indica","dates":{"release":"2008-01-01T00:00:00Z","publication":"2008 Aug","modification":"2025-05-31T23:50:35.497Z","creation":"2019-06-06T19:31:13Z"},"accession":"S-EPMC2519352","cross_references":{"pubmed":["18515483"],"doi":["10.1128/aem.00623-08","10.1128/AEM.00623-08"]}}